Duman Muhammed, Mulet Magdalena, Altun Soner, Saticioglu Izzet Burcin, Gomila Margarita, Lalucat Jorge, Garcia-Valdes Elena
Department of Aquatic Animal Diseases, Bursa Uludag University, Faculty of Veterinary Medicine, 16059 Bursa, Turkey.
Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004714. Epub 2021 Feb 25.
Six Gram negative, motile bacteria were isolated from rainbow trout (). The 16S rRNA sequence similarity values grouped them in the (strains P49, P50, 154a and P154b), (strain P115) and (strain P155) phylogenetic subgroups in the genus . The DNA G+C content ranged from 58.5 to 60 mol%. The strains were characterized phenotypically using API 20NE and Biolog GENIII tests, and chemotaxonomically by their whole-cell MALDI-TOF MS protein profiles and fatty acid contents. Multi-locus sequence analysis with four housekeeping gene sequences (, , and 16S rRNA) together with genome comparisons by average nucleotide identity and genome-to-genome distance calculations were performed. Results showed that the similarity values of these strains to known species type strains were lower than the thresholds established for species in the genus . Based on these data, we concluded that strains P49, P50, P115, P154a, P154b and P155 belonged to four novel species. The names proposed are: sp. nov. for strains P49 and P50 with P50 (=CECT 30175=CCUG 74871) as the type strain; sp. nov. for strain P115 (CECT 30173=CCUG 74873) sp. nov. for strains P154a and P154b with P154a (=CECT 30177=CCUG 74874) as the type strain; and sp. nov. for strain P155 (=CECT 30178=CCUG 74875).
从虹鳟鱼中分离出6株革兰氏阴性、具运动性的细菌。16S rRNA序列相似性值将它们归为气单胞菌属的嗜水气单胞菌（菌株P49、P50、154a和P154b）、温和气单胞菌（菌株P115）和豚鼠气单胞菌（菌株P155）系统发育亚组。DNA G+C含量范围为58.5至60摩尔%。使用API 20NE和Biolog GENIII测试对菌株进行表型特征分析，并通过全细胞MALDI-TOF MS蛋白质谱和脂肪酸含量进行化学分类分析。进行了四个管家基因序列（gyrB、rpoD、recA和16S rRNA）的多位点序列分析以及通过平均核苷酸同一性和基因组到基因组距离计算进行的基因组比较。结果表明，这些菌株与已知物种模式菌株的相似性值低于气单胞菌属中为物种确定的阈值。基于这些数据，我们得出结论，菌株P49、P50、P115、P154a、P154b和P155属于四个新物种。提议的名称为：以P50（=CECT 30175=CCUG 74871）为模式菌株的P49和P50的嗜水气单胞菌新种；以菌株P115（CECT 30173=CCUG 74873）为模式菌株的温和气单胞菌新种；以P154a（=CECT 30177=CCUG 74874）为模式菌株的P154a和P154b的豚鼠气单胞菌新种；以及以菌株P155（=CECT 30178=CCUG 74875）为模式菌株的豚鼠气单胞菌新种。
