University "G. d'Annunzio" of Chieti-Pescara, Department of Advanced Technologies in Medicine & Dentistry, Chieti, Italy.
Center for Advanced Studies and Technology (CAST), University "G. d'Annunzio" of Chieti-Pescara, Chieti, Italy.
J Med Microbiol. 2021 Mar;70(3). doi: 10.1099/jmm.0.001328. Epub 2021 Feb 25.
This study tests the release of SARS-CoV-2 RNA into the air during normal breathing, without any sign of possible risk of contagion such as coughing, sneezing or talking. Five patients underwent oropharyngeal, nasopharyngeal and salivary swabs for real-time reverse transcriptase PCR (RT-PCR) detection of SARS-CoV-2 RNA. Direct SARS-CoV-2 release during normal breathing was also investigated by RT-PCR in air samples collected using a microbiological sampler. Viral RNA was detected in air at 1 cm from the mouth of patients whose oropharyngeal, nasopharyngeal and salivary swabs tested positive for SARS-CoV-2 RNA. In contrast, the viral RNA was not identified in the exhaled air from patients with oropharyngeal, nasopharyngeal and salivary swabs that tested negative. Contagion of SARS-CoV-2 is possible by being very close to the mouth of someone who is infected, asymptomatic and simply breathing.
这项研究测试了在没有任何可能的传染风险迹象(如咳嗽、打喷嚏或说话)的情况下,正常呼吸时 SARS-CoV-2 病毒 RNA 释放到空气中的情况。五名患者接受了口咽、鼻咽和唾液拭子,以进行实时逆转录酶 PCR(RT-PCR)检测 SARS-CoV-2 RNA。通过使用微生物采样器收集的空气样本中的 RT-PCR 也研究了正常呼吸时 SARS-CoV-2 的直接释放情况。在距离检测出 SARS-CoV-2 RNA 阳性的患者口部 1 厘米处的空气中检测到了病毒 RNA。相比之下,在咽、鼻和唾液拭子检测结果为 SARS-CoV-2 RNA 阴性的患者的呼出空气中,并未识别出病毒 RNA。与感染 SARS-CoV-2 的无症状感染者近距离接触,即使感染者只是在正常呼吸,也可能会感染 SARS-CoV-2。