厄瓜多尔 COVID-19 人群血清学状态检测的 SARS-CoV-2 rRBD ELISA 试剂盒：一种低成本方法
A Low-Cost SARS-CoV-2 rRBD ELISA to Detect Serostatus in Ecuadorian Population with COVID-19.
机构信息
1Instituto de Biomedicina, Carrera de Medicina, Universidad Central, Quito, Ecuador.
2Distrito de Salud 09D13, Ministerio de Salud Pública del Ecuador, Balzar, Ecuador.
出版信息
Am J Trop Med Hyg. 2021 Feb 25;104(4):1513-1515. doi: 10.4269/ajtmh.20-1420.
Laboratory diagnosis of the COVID-19 relies on RT-PCR to amplify specific fragments of SARS-CoV-2 genome. However, serological tests are required to determine the immune response elicited after infection. Here, we analyzed convalescent sera collected from positive individuals by RT-PCR to SARS-CoV-2 (n = 78), Zika (n = 20), dengue (n = 20), chikungunya (n = 54), intestinal parasites (n = 11), and HIV (n = 1), from different areas of Ecuador, with an in-house ELISA using a SARS-CoV-2 receptor binding domain recombinant (rRBD) antigen to detect IgG antibodies elicited by SARS-CoV-2 infection. Of the 78 samples positive for SARS-CoV-2 by RT-PCR, 73 showed high absorbance value compared with the cutoff and five were negative. All tested sera from other infections showed no reactivity. Sensitivity, specificity, positive predictive value, and negative predictive value were 93.6%, 100%, 100%, and 95.4%, respectively. This in-house anti-IgG rRBD ELISA offers an economic and simple alternative to determine IgG immune responses after SARS-CoV-2 infection.
实验室诊断 COVID-19 依赖于 RT-PCR 来扩增 SARS-CoV-2 基因组的特定片段。然而，需要血清学检测来确定感染后产生的免疫反应。在这里，我们分析了从厄瓜多尔不同地区通过 RT-PCR 检测为阳性的个体的恢复期血清（n = 78），包括 SARS-CoV-2（n = 78）、寨卡病毒（n = 20）、登革热（n = 20）、基孔肯雅热（n = 54）、肠道寄生虫（n = 11）和 HIV（n = 1），使用内部 ELISA 检测 SARS-CoV-2 受体结合域重组（rRBD）抗原，以检测 SARS-CoV-2 感染引起的 IgG 抗体。在通过 RT-PCR 检测为 SARS-CoV-2 阳性的 78 个样本中，73 个样本的吸光度值高于临界值，5 个样本为阴性。所有来自其他感染的测试血清均无反应。该方法的灵敏度、特异性、阳性预测值和阴性预测值分别为 93.6%、100%、100%和 95.4%。这种内部 IgG rRBD ELISA 为确定 SARS-CoV-2 感染后的 IgG 免疫反应提供了一种经济且简单的替代方法。
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