Cheng Jie, Tu Wenying, Luo Zhou, Gou Xinghua, Li Qiang, Wang Dan, Zhou Jingwen
Key Laboratory of Meat Processing of Sichuan Province, Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, College of Food and Biological Engineering, Chengdu University, Chengdu, China.
Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China.
Front Bioeng Biotechnol. 2021 Feb 9;9:633028. doi: 10.3389/fbioe.2021.633028. eCollection 2021.
Bioproduction of 5-aminovalerate (5AVA) from renewable feedstock can support a sustainable biorefinery process to produce bioplastics, such as nylon 5 and nylon 56. In order to achieve the biobased production of 5AVA, a 2-keto-6-aminocaproate-mediated synthetic pathway was established. Combination of L-Lysine α-oxidase from , α-ketoacid decarboxylase from and aldehyde dehydrogenase from could achieve the biosynthesis of 5AVA from biobased L-Lysine in . The HO produced by L-Lysine α-oxidase was decomposed by the expression of catalase KatE. Finally, 52.24 g/L of 5AVA were obtained through fed-batch biotransformation. Moreover, homology modeling, molecular docking and molecular dynamic simulation analyses were used to identify mutation sites and propose a possible trait-improvement strategy: the expanded catalytic channel of mutant and more hydrogen bonds formed might be beneficial for the substrates stretch. In summary, we have developed a promising artificial pathway for efficient 5AVA synthesis.
从可再生原料生物生产5-氨基戊酸（5AVA）可支持可持续生物精炼过程，以生产生物塑料，如尼龙5和尼龙66。为了实现5AVA的生物基生产，建立了一条2-酮-6-氨基己酸介导的合成途径。来自[具体来源1]的L-赖氨酸α-氧化酶、来自[具体来源2]的α-酮酸脱羧酶和来自[具体来源3]的醛脱氢酶相结合，可在[具体体系]中从生物基L-赖氨酸实现5AVA的生物合成。L-赖氨酸α-氧化酶产生的H₂O₂通过过氧化氢酶KatE的表达得以分解。最后，通过补料分批生物转化获得了52.24 g/L的5AVA。此外，利用同源建模、分子对接和分子动力学模拟分析来确定突变位点并提出可能的性状改善策略：突变体扩大的催化通道和形成的更多氢键可能有利于底物伸展。总之，我们开发了一条有前景的高效合成5AVA的人工途径。
