Cheng Jie, Tu Wenying, Luo Zhou, Liang Li, Gou Xinghua, Wang Xinhui, Liu Chao, Zhang Guoqiang
Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Front Bioeng Biotechnol. 2021 Sep 16;9:726126. doi: 10.3389/fbioe.2021.726126. eCollection 2021.
The compounds 5-aminovalerate and δ-valerolactam are important building blocks that can be used to synthesize bioplastics. The production of 5-aminovalerate and δ-valerolactam in microorganisms provides an ideal source that reduces the cost. To achieve efficient biobased coproduction of 5-aminovalerate and δ-valerolactam in , a single biotransformation step from L-lysine was constructed. First, an equilibrium mixture was formed by L-lysine α-oxidase RaiP from . In addition, by adjusting the pH and HO concentration, the titers of 5-aminovalerate and δ-valerolactam reached 10.24 and 1.82 g/L from 40 g/L L-lysine HCl at pH 5.0 and 10 mM HO, respectively. With the optimized pH value, the δ-valerolactam titer was improved to 6.88 g/L at pH 9.0 with a molar yield of 0.35 mol/mol lysine. The ratio of 5AVA and δ-valerolactam was obviously affected by pH value. The ratio of 5AVA and δ-valerolactam could be obtained in the range of 5.63:1-0.58:1 at pH 5.0-9.0 from the equilibrium mixture. As a result, the simultaneous synthesis of 5-aminovalerate and δ-valerolactam from L-lysine in is highly promising. To our knowledge, this result constitutes the highest δ-valerolactam titer reported by biological methods. In summary, a commercially implied bioprocess developed for the coproduction of 5-aminovalerate and δ-valerolactam using engineered .
化合物5-氨基戊酸和δ-戊内酰胺是重要的结构单元，可用于合成生物塑料。微生物中5-氨基戊酸和δ-戊内酰胺的生产提供了一种降低成本的理想来源。为了在[具体微生物]中实现5-氨基戊酸和δ-戊内酰胺的高效生物基联产，构建了一个从L-赖氨酸开始的单一生物转化步骤。首先，由[具体微生物]的L-赖氨酸α-氧化酶RaiP形成一个平衡混合物。此外，通过调节pH值和过氧化氢浓度，在pH 5.0和10 mM过氧化氢条件下，从40 g/L L-赖氨酸盐酸盐中5-氨基戊酸和δ-戊内酰胺的产量分别达到10.24 g/L和1.82 g/L。在优化的pH值下，在pH 9.0时δ-戊内酰胺产量提高到6.88 g/L，摩尔产率为0.35 mol/mol赖氨酸。5-氨基戊酸（5AVA）和δ-戊内酰胺的比例明显受pH值影响。在pH 5.0 - 9.0条件下，从平衡混合物中可获得5AVA与δ-戊内酰胺的比例范围为5.63:1 - 0.58:1。因此，在[具体微生物]中从L-赖氨酸同时合成5-氨基戊酸和δ-戊内酰胺具有很大的前景。据我们所知，这一结果构成了通过生物方法报道的最高δ-戊内酰胺产量。总之，利用工程化的[具体微生物]开发了一种用于5-氨基戊酸和δ-戊内酰胺联产的具有商业潜力的生物工艺。
