Utilization of a Wheat Sidestream for 5-Aminovalerate Production in .

Production of plastics from petroleum-based raw materials extensively contributes to global pollution and CO emissions. Biotechnological production of functionalized monomers can reduce the environmental impact, in particular when using industrial sidestreams as feedstocks. , which is used in the million-ton-scale amino acid production, has been engineered for sustainable production of polyamide monomers. In this study, wheat sidestream concentrate (WSC) from industrial starch production was utilized for production of l-lysine-derived bifunctional monomers using metabolically engineered strains. Growth of on WSC was observed and could be improved by hydrolysis of WSC. By heterologous expression of the genes ( from ) and from , xylose, and arabinose in WSC hydrolysate (WSCH), in addition to glucose, could be consumed, and production of l-lysine could be increased. WSCH-based production of cadaverine and 5-aminovalerate (5AVA) was enabled. To this end, the lysine decarboxylase gene from was expressed alone or for conversion to 5AVA cascaded either with putrescine transaminase and dehydrogenase genes from or with putrescine oxidase gene from and . Deletion of the l-glutamate dehydrogenase-encoding gene reduced formation of l-glutamate as a side product for strains with either of the cascades. Since the former cascade ( - ) yields l-glutamate, 5AVA production is coupled to growth by flux enforcement resulting in the highest 5AVA titer obtained with WSCH-based media.