Department of Chemistry, Faculty of Science and Letters, Manisa Celal Bayar University, Yağcilar Mahallesi, 45140, Yunusemre, Manisa-Turkey, Manisa, Turkey.
Department of Medicinal and Aromatic Plants, Giresun University, Espiye 28600, Espiye/Giresun-Turkey, Giresun, Turkey.
J Chromatogr Sci. 2021 May 20;59(6):491-501. doi: 10.1093/chromsci/bmab022.
Investigation of phenolic content from different pine bark species grown in Turkey was performed using a reversed-phase high pressure liquid chromatography with ultraviolet (RP-HPLC-UV) method. All phenolic constituents were separated in <26 min on reversed-phase C18 column with gradient mobile phase that consists of orthophosphoric acid, methanol and acetonitrile. Detections were made on an UV detector at 280 nm and at a flow rate of 1 mL/min. Samples were prepared according to Masqueller's conventional sample preparation method with slight modifications. To avoid the reduction in extraction efficiency the sample preparation step was carried out under argon atmosphere. The linearity of the method was between 0.9994 and 0.9999. The detection limits for the five phenolic constituents ranged from 0122 to 0.324 mg/L. Catechin and taxifolin were found in all pine barks at a concentration of 0.065 ± 0.002-1.454 ± 0.004 and 0.015 ± 0.001-23.164 ± 0.322 mg/g, respectively. Epicatechin was determined in four pine barks between 0.027 ± 0.001 and 0.076 ± 0.002 mg/g, ferulic acid in two pine barks between 0.010 ± 0.001 and 0.022 ± 0.001 mg/g and epicatechin gallate in only one of the pine barks at 0.025 ± 0.001 mg/g. Finally, the total amount of phenolic compounds and antioxidant capacities of the pine barks were found to be very high.
采用反相高效液相色谱法（RP-HPLC-UV），对土耳其不同松属植物皮中的酚类含量进行了研究。在反相 C18 柱上，采用含有正磷酸、甲醇和乙腈的梯度流动相，可在 26 分钟内分离所有酚类成分。在 280nm 的紫外检测器和 1ml/min 的流速下进行检测。样品按照 Masqueller 的常规样品制备方法进行制备，略有修改。为避免萃取效率降低，样品制备步骤在氩气气氛下进行。该方法的线性范围在 0.9994 到 0.9999 之间。五种酚类成分的检测限范围为 0.122 到 0.324mg/L。在所有的松皮中均发现了儿茶素和松脂素，浓度分别为 0.065±0.002-1.454±0.004 和 0.015±0.001-23.164±0.322mg/g。在四种松皮中测定了表儿茶素，浓度在 0.027±0.001 和 0.076±0.002mg/g 之间，在两种松皮中测定了阿魏酸，浓度在 0.010±0.001 和 0.022±0.001mg/g 之间，在一种松皮中仅测定了表儿茶素没食子酸酯，浓度为 0.025±0.001mg/g。最后，发现松皮中的总酚类化合物和抗氧化能力非常高。
