Chemistry Laboratory and methodology for Environment, Paul-Verlaine University, boulevard Arago, Metz, France.
C R Biol. 2010 Sep;333(9):663-9. doi: 10.1016/j.crvi.2010.07.002. Epub 2010 Aug 23.
The present study was carried out to evaluate the antioxidant activities of the root bark extract of Securidaca longepedunculata. This plant material is commonly used in folk medicine in several parts in the world. The bark extracts of S. longepedunculata were evaluated for their total phenols, flavonoids, anthocyanins, tannins content and total antioxidant capacity. The compounds were identified and quantified both by RP-HPLC and UV spectrophotometer; the antioxidant capacity was assessed by ABTS and DPPH tests and expressed as IC(50). The total phenolic compounds determinate was 9.86mg gallic acid equivalents/g dw, the total flavonoid contents was 5.85mg catechin equivalents/g dw, the total anthocyanin contents was 0.032mg cyanidin-3-glycosyl equivalents/g dw and the condensed tannins content were 1.03mg catechin equivalents/g dw. The major compound identified using RP-HPLC was quercetin (0.98mg/ml). The IC(50) value reached 5.5microg/ml, revealing that the root barks of S. longepedunculata have a very high antioxidant and anti-inflammation properties.
本研究旨在评估长柄翅巴戟根皮提取物的抗氧化活性。这种植物材料在世界上的几个地方都被民间医学广泛使用。对 S. longepedunculata 的树皮提取物进行了总酚、类黄酮、花青素、单宁含量和总抗氧化能力的评估。通过反相高效液相色谱法（RP-HPLC）和紫外分光光度计对化合物进行了鉴定和定量；通过 ABTS 和 DPPH 试验评估了抗氧化能力，并以 IC（50）表示。总酚类化合物的测定值为 9.86mg 没食子酸当量/gdw，总类黄酮含量为 5.85mg 儿茶素当量/gdw，总花青素含量为 0.032mg 矢车菊素-3-葡萄糖苷当量/gdw，缩合单宁含量为 1.03mg 儿茶素当量/gdw。使用 RP-HPLC 鉴定的主要化合物是槲皮素（0.98mg/ml）。IC（50）值达到 5.5μg/ml，表明长柄翅巴戟根皮具有很高的抗氧化和抗炎特性。
