Kim Ye-Jun, Nam Tae Gyu, Lee Inil, Heo Ho Jin, Kim Dae-Ok
Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 17104 Republic of Korea.
Major of Food Science and Biotechnology, Division of Bio-Convergence, Kyonggi University, Suwon, 16227 Republic of Korea.
Food Sci Biotechnol. 2024 Jul 8;34(5):1139-1148. doi: 10.1007/s10068-024-01616-x. eCollection 2025 Mar.
Pine () bark, most of which is discarded as a by-product of the timber industry, contains considerable amounts of antioxidant phenolics. We developed and validated a quantitative method of analyzing seven phenolics (protocatechuic acid, procyanidin B1, procyanidin B3, catechin, ferulic acid, taxifolin, and quercetin) in pine bark extract (PBE) using a high-performance liquid chromatography-photodiode array. The simultaneous analytical method for the seven phenolics was validated in terms of specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision. The correlation coefficients of the calibration curves of the seven phenolics were ≥0.9999. The LODs and LOQs of the seven phenolics were 0.01-0.16 and 0.02-0.49 μg/mL, respectively. The ranges of recoveries of the seven phenolics were 97.29-103.59%, and their relative standard deviations were 0.24-3.95%. The described approach to the quantitative analysis of PBE phenolics as marker compounds can be used for standardization in the development of health-functional foods.
The online version contains supplementary material available at 10.1007/s10068-024-01616-x.
松树树皮大多作为木材工业的副产品被丢弃，但其含有大量抗氧化酚类物质。我们开发并验证了一种使用高效液相色谱 - 光电二极管阵列分析松树皮提取物（PBE）中七种酚类物质（原儿茶酸、原花青素B1、原花青素B3、儿茶素、阿魏酸、紫杉叶素和槲皮素）的定量方法。对这七种酚类物质的同时分析方法在特异性、线性、检测限（LOD）、定量限（LOQ）、准确度和精密度方面进行了验证。七种酚类物质校准曲线的相关系数均≥0.9999。七种酚类物质的检测限和定量限分别为0.01 - 0.16和0.02 - 0.49μg/mL。七种酚类物质的回收率范围为97.29 - 103.59%，其相对标准偏差为0.24 - 3.95%。所描述的将PBE酚类物质作为标记化合物进行定量分析的方法可用于健康功能食品开发中的标准化。
在线版本包含可在10.1007/s10068 - 024 - 01616 - x获取的补充材料。
