Department of Transfusion Medicine, Tokyo Metropolitan Bokutoh Hospital, Tokyo, Japan.
Clinical Laboratory, Tokyo Metropolitan Bokutoh Hospital, Tokyo, Japan.
Transfusion. 2021 Apr;61(4):1035-1040. doi: 10.1111/trf.16331. Epub 2021 Feb 26.
Cryoprecipitate (CRYO) is neither produced nor supplied by the Japanese Red Cross Society. A novel CRYO extraction method established in-house by modifying a thaw-siphon technique was demonstrated in this study.
A pack of fresh frozen plasma was thawed and equally divided into two bags for CRYO extraction by different methods. CRYO was extracted from the blood plasma using a standard centrifugation method and our modified thaw-siphon method (Bokutoh-siphon method; B method). The two different CRYOs extracted were analyzed to compare the differences in the amount of fibrinogen recovered, clotting factors extracted, and clotting activity.
The amount of fibrinogen in the CRYO extracted using the B-siphon method was similar to that obtained using the standard method (recovery of fibrinogen: B-siphon method: 71.2% vs. standard method: 61.0%). The amount of clotting XIII factor extracted using the B-siphon method was significantly lower than those extracted using the standard method. On the other hand, clotting II, V factors, and C1q esterase inhibitor not concentrated in CRYO content from the B-siphon method were significantly higher than that from the standard method.
A new in-house CRYO preparation method was established by modifying a previously used thaw-siphon method. A coagulation factor-rich CRYO was extracted from plasma frozen at -40°C along with the first fraction of thawed plasma, without using a large-capacity refrigerated centrifuge for blood bags.
日本红十字会既不生产也不供应冷沉淀（CRYO）。本研究中，通过对解冻虹吸技术进行改良，建立了一种新的冷沉淀提取方法。
将一包新鲜冷冻血浆解冻并等分两份，通过不同的方法进行冷沉淀提取。使用标准离心法和我们改良的解冻虹吸法（Bokutoh-siphon 法；B 法）从血浆中提取冷沉淀。分析两种不同的冷沉淀，比较回收的纤维蛋白原量、提取的凝血因子量和凝血活性的差异。
使用虹吸法提取的冷沉淀中的纤维蛋白原量与使用标准方法提取的纤维蛋白原量相似（纤维蛋白原回收率：虹吸法：71.2% vs. 标准法：61.0%）。使用虹吸法提取的凝血 XIII 因子量明显低于使用标准方法提取的量。另一方面，从虹吸法提取的冷沉淀中未浓缩的凝血 II、V 因子和 C1q 酯酶抑制剂的量明显高于标准法。
通过改良先前使用的解冻虹吸法，建立了一种新的内部冷沉淀制备方法。从-40°C 冷冻的血浆中，与第一份解冻的血浆一起，提取了富含凝血因子的冷沉淀，而无需使用大容量冷藏离心机处理血袋。
