评估用于冷沉淀生产的“梅森”（连续解冻虹吸）法。

Evaluation of the 'Mason' (continuous-thaw-siphon) method for cryoprecipitate production.

作者信息

Prowse C V, McGill A

出版信息

Vox Sang. 1979;37(4):235-43. doi: 10.1111/j.1423-0410.1979.tb02297.x.

DOI:10.1111/j.1423-0410.1979.tb02297.x

Abstract

A comparison was made of the factor VIII recovery in cryoprecipitate made by the continuous-thaw-siphon technique of Mason [1], by our routine method of overnight thawing at 4 degrees C and by a fast-thaw method. The siphon method resulted in a mean recovery of 71% of the factor VIII. This is at least double the yield obtained by the routine method and could not be solely attributed to the faster processing as cryoprecipitate prepared by the fast-thaw method gave a factor VIII yield of 53%, which was significantly less than the Mason product. This product also contained increased amounts of factor VIII-related antigen and fibrinogen. Characterisation of the thaw-siphon cryoprecipitate factor VII by gel filtration, electrophoretic mobility, stability and measurement of isoagglutinin titre did not reveal any significant qualitative differences from cryoprecipitate produced by other methods.

摘要

对采用梅森[1]的连续解冻虹吸技术制备的冷沉淀、我们在4摄氏度过夜解冻的常规方法以及快速解冻法所制得的冷沉淀中的凝血因子VIII回收率进行了比较。虹吸法的凝血因子VIII平均回收率为71%。这至少是常规方法所得产量的两倍，且不能仅仅归因于处理速度更快，因为快速解冻法制备的冷沉淀的凝血因子VIII产量为53%，明显低于梅森法的产品。该产品还含有增加量的凝血因子VIII相关抗原和纤维蛋白原。通过凝胶过滤、电泳迁移率、稳定性以及同种凝集素滴度测定对解冻虹吸冷沉淀凝血因子VII进行表征，未发现与其他方法制备的冷沉淀有任何显著的质量差异。