Mason E C
Lancet. 1978 Jul 1;2(8079):15-7. doi: 10.1016/s0140-6736(78)91324-7.
Plasma, which has been deep-frozen in plastic packs as flat slabs, is thawed in a 3-5 degrees C water-bath, and allowed to siphon continually into an attached satellite pack external to the bath. This thaw-siphon procedure is completed within 55-65 min and the cryoprecipitate, retained in the residual 25 ml of frozen plasma, contains 70-100% of the original plasma factor VIII. Clinical observations in haemophilic patients treated with these cryoprecipitates, have confirmed that the higher in-vitro content of factor VIII is associated with enhanced in-vivo effectiveness.
血浆以扁平板状深冻于塑料包装中，在3 - 5摄氏度的水浴中解冻，并持续虹吸到水浴外连接的卫星袋中。这种解冻-虹吸过程在55 - 65分钟内完成，保留在剩余25毫升冷冻血浆中的冷沉淀含有原始血浆中70 - 100%的凝血因子VIII。对接受这些冷沉淀治疗的血友病患者的临床观察证实，较高的体外凝血因子VIII含量与增强的体内疗效相关。
