Complex hydroperiod induced carryover responses for survival, growth, and endurance of a pond-breeding amphibian.

Assessing carryover effects from the aquatic to the terrestrial stage of pond-breeding amphibians is critical as temperature and hydrologic regimes of temporary ponds continue to be altered as a result of climate change and other stressors. We evaluated carryover effects of hydroperiod length (50-62 days) on amphibian survival, developmental rates, and locomotor performance using a model organism, the wood frog (Rana sylvatica), through aquatic and terrestrial mesocosm experiments with individual tests of locomotor performance. We found that shorter hydroperiods (50 days) had low larval survival (0.44 ± 0.03) compared to the 62-day hydroperiod (0.91 ± 0.09) and increased developmental rates, resulting in smaller sizes at metamorphosis. We did not find evidence of carryover effects on terrestrial survival three months post-metamorphosis with all hydroperiod treatments showing high terrestrial survival (0.88 ± 0.07). However, post-metamorphic frogs from the longer hydroperiod treatments grew faster and larger compared to individuals from shortest hydroperiods and performed significantly better during endurance trials at 18 °C. Disentangling complex carryover effects across multiple life stages in species with high phenotypic plasticity can shed light on the physiological capacity of species to respond to changing environments and inform mechanistic predictions of persistence in the face of anthropogenic stressors.