Matsumoto M, Hatakeyama T, Akai F, Brengman J M, Yanagihara T
Department of Neurology, Mayo Clinic, Rochester, MN 55905.
Stroke. 1988 Apr;19(4):490-7. doi: 10.1161/01.str.19.4.490.
A method was developed to predict the severity of cerebral ischemia before permanent occlusion of a common carotid artery in gerbils by observing the diameter and appearance of the artery after temporary occlusion and observing clinical signs after permanent occlusion. The severity of cerebral ischemia was confirmed by a sensitive immunohistochemical method and measurement of focal cerebral blood flow after 30 minutes' ischemia. All gerbils with greater than 40% reduction of the diameter and a white arterial margin distal to temporary occlusion developed severe neurologic signs following permanent occlusion, but no gerbils with reduction of less than 30% and a red arterial margin developed neurologic signs. With the cumulative neurologic score, gerbils could be divided into classes with no, mild, moderate, and severe symptoms, mostly after 10 minutes. Severely symptomatic gerbils were identified in 3 minutes. Extensive ischemic damage was observed in severely symptomatic gerbils, but no immunohistochemical lesion was detected in mildly symptomatic gerbils. Cerebral blood flow was markedly reduced in severely symptomatic gerbils but more selectively reduced in the cortical structures of moderately symptomatic gerbils. This prediction method is useful for investigating early cerebral ischemia and for evaluating the effectiveness of pharmacologic agents.
通过观察沙土鼠颈总动脉暂时阻断后动脉的直径和外观以及永久阻断后的临床体征，开发了一种在沙土鼠颈总动脉永久阻断前预测脑缺血严重程度的方法。通过敏感的免疫组织化学方法和缺血30分钟后局部脑血流量的测量来确认脑缺血的严重程度。所有暂时阻断后动脉直径减少超过40%且远端动脉边缘呈白色的沙土鼠在永久阻断后均出现严重神经体征，但动脉直径减少少于30%且动脉边缘呈红色的沙土鼠均未出现神经体征。根据累积神经评分，沙土鼠大多在10分钟后可分为无症状、轻度、中度和重度症状组。在3分钟内即可识别出有严重症状的沙土鼠。在有严重症状的沙土鼠中观察到广泛的缺血性损伤，但在有轻度症状的沙土鼠中未检测到免疫组织化学损伤。有严重症状的沙土鼠脑血流量明显减少，但有中度症状的沙土鼠皮质结构中的脑血流量减少更具选择性。这种预测方法有助于研究早期脑缺血和评估药物的有效性。
