Kitagawa K, Matsumoto M, Tagaya M, Ueda H, Oku N, Kuwabara K, Ohtsuki T, Handa N, Kimura K, Kamada T
First Department of Internal Medicine, Osaka University Medical School, Japan.
Acta Neuropathol. 1991;82(3):164-71. doi: 10.1007/BF00294441.
We investigated the temporal profile of the extravasation of serum albumin in a reproducible gerbil model of unilateral cerebral ischemia, using immunohistochemical and dye-tracer techniques to evaluate albumin accumulation and the occurrence of active extravasation, respectively. After 30 min of cerebral ischemia and subsequent reperfusion, immunostaining for albumin became visible in the lateral part of the thalamus during the first 3 h, and then expanded to other brain regions up to 24 h. At both 24 h and 3 days after reperfusion, massive extravasation of albumin was noted in the whole ischemic hemisphere, and this had decreased again by 7 days after reperfusion. The extent and the degree of albumin immunopositivity were almost the same in all animals examined at each period after reperfusion. The extravasation of Evans blue, which was allowed to circulate for 30 min before death, was limited to the lateral part of the thalamus during the first 6 h of reperfusion. In the circumscribed area of massive albumin extravasation, many neurons were immunopositive for albumin; most of these neurons appeared to be intact and also showed immunostaining for microtubule-associated protein 2. The current investigation clearly demonstrated that (1) albumin extravasation was produced with reliable reproducibility in this model, (2) the lateral part of the thalamus was the region most vulnerable to ischemic blood-brain barrier damage, and (3) many apparently intact neurons in the ischemic region were positive for albumin.
我们在可重复的单侧脑缺血沙鼠模型中，利用免疫组织化学和染料示踪技术分别评估白蛋白的蓄积情况及活性外渗的发生情况，研究了血清白蛋白外渗的时间进程。脑缺血30分钟并随后再灌注后，在最初3小时内，丘脑外侧部可见白蛋白免疫染色，随后扩展至其他脑区，直至24小时。再灌注后24小时和3天时，整个缺血半球均出现大量白蛋白外渗，而再灌注7天时这种情况再次减少。在再灌注后各时间段检查的所有动物中，白蛋白免疫阳性的范围和程度几乎相同。伊文思蓝在死亡前循环30分钟，其外渗在再灌注的最初6小时内局限于丘脑外侧部。在大量白蛋白外渗的限定区域，许多神经元白蛋白免疫阳性；这些神经元中的大多数似乎完好无损，并且微管相关蛋白2也呈免疫染色阳性。本研究清楚地表明：（1）在该模型中白蛋白外渗具有可靠的可重复性；（2）丘脑外侧部是最易受缺血性血脑屏障损伤的区域；（3）缺血区域许多明显完好的神经元白蛋白呈阳性。
