Yusuf Mohammad, Saeed Almehrzi Alia S, Nasir Alnajjar Alya J, Alam Pravej, Elsayed Nesma, Khalil Radwan, Hayat Shamsul
Department of Biology, College of Science, United Arab Emirates University, Al Ain, 15551, United Arab Emirates.
Department of Biology, College of Science, United Arab Emirates University, Al Ain, 15551, United Arab Emirates.
Carbohydr Res. 2021 Mar;501:108271. doi: 10.1016/j.carres.2021.108271. Epub 2021 Feb 19.
Glucose is recognized as signaling molecule that regulates growth and development of plants under various environmental cues, but their effect in regulation of copper induced toxicity in plants is not yet investigated. This study revealed the effect of exogenously sourced glucose on Cucumber plants exposed to increasing concentration of copper. Glucose mediated response on growth performance, photosynthetic efficiency, antioxidant enzymes, oxidative stress markers, ion uptake were analyzed in the presence and absence of copper. Glucose alone and in combination with lower concentration of copper improved the growth, photosynthetic performance, and antioxidant capacity of cucumber plants. However, higher concentrations of copper alone showed oxidative damage through increased electrolyte leakage, HO accumulation, lipid peroxidation and reduced uptake of macronutrients. Application of glucose to copper-stressed plants enhanced activities of Rubisco, antioxidant enzymes, proline accumulation and maintained copper level in aerial parts of plants. These enhanced activities of antioxidant enzymes, proline accumulation, uptake of NPK and maintained equilibrium of copper in plants, leading to detoxification of copper stress in cucumber plants. This study provides an understanding that exogenous application of glucose can be employed as vital biochemical approach in alleviating copper-induced toxicity and could be utilized as phytoremediation technique for removal of excess transition metal from polluted soil.
葡萄糖被认为是一种信号分子，可在各种环境线索下调节植物的生长和发育，但其在调节植物铜诱导毒性方面的作用尚未得到研究。本研究揭示了外源葡萄糖对暴露于不断增加铜浓度下的黄瓜植株的影响。在有铜和无铜的情况下，分析了葡萄糖介导的对生长性能、光合效率、抗氧化酶、氧化应激标志物、离子吸收的反应。单独的葡萄糖以及与较低浓度铜组合使用时，可改善黄瓜植株的生长、光合性能和抗氧化能力。然而，单独使用较高浓度的铜会通过增加电解质渗漏、羟基积累、脂质过氧化以及减少大量营养素的吸收而表现出氧化损伤。向受铜胁迫的植株施用葡萄糖可增强核酮糖-1,5-二磷酸羧化酶（Rubisco）、抗氧化酶的活性，促进脯氨酸积累，并维持植株地上部分的铜水平。抗氧化酶活性的增强、脯氨酸的积累、氮磷钾的吸收以及植株中铜的平衡维持，导致黄瓜植株铜胁迫的解毒。本研究表明，外源施用葡萄糖可作为缓解铜诱导毒性的重要生化方法，并可作为从污染土壤中去除过量过渡金属的植物修复技术。
