Chmielowska-Bąk Jagna, Shcheglova Ekaterina, Rosik Konrad, Yushin Nikita, Zinicovscaia Inga, Deckert Joanna
Department of Plant Ecophysiology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, Poznań, Poland.
Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Russia.
Front Plant Sci. 2022 Jan 31;12:828620. doi: 10.3389/fpls.2021.828620. eCollection 2021.
Plant exposure to metals is associated with the accumulation of reactive oxygen species, which mediate the oxidation of various molecules including lipids, proteins, and nucleic acids. The aim of the present study is the evaluation of the impact of short-term Cu and Pb treatment on oxidative events in the roots of soybean seedlings, with special emphasis on RNA oxidation. The results show that an increase in total RNA oxidative modification, 8-hydroxyguanosine (8-OHG), constitutes a very early response to both applied metals, observed already within the first hour of treatment. Exposure to Cu and Pb resulted also in the increase in superoxide anion and hydrogen peroxide levels and intensified lipid peroxidation. However, these responses were most prominent after longer treatment times. On the other hand, no changes were observed in the level of protein carbonylation. It can be concluded that 8-OHG enrichment in total RNA constitutes one of the earliest reactions to metals, which precedes the symptoms of oxidative stress.
植物接触金属与活性氧的积累有关，活性氧介导包括脂质、蛋白质和核酸在内的各种分子的氧化。本研究的目的是评估短期铜和铅处理对大豆幼苗根系氧化事件的影响，特别关注RNA氧化。结果表明，总RNA氧化修饰产物8-羟基鸟苷（8-OHG）的增加是对两种施用金属的非常早期的反应，在处理的第一小时内就已观察到。暴露于铜和铅还导致超氧阴离子和过氧化氢水平升高以及脂质过氧化加剧。然而，这些反应在较长处理时间后最为明显。另一方面，蛋白质羰基化水平未观察到变化。可以得出结论，总RNA中8-OHG的富集是对金属的最早反应之一，早于氧化应激症状。
