一种与动力蛋白相关的光感受器蛋白可防止纤毛适应蓝光。
A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light.
作者信息
Kutomi Osamu, Yamamoto Ryosuke, Hirose Keiko, Mizuno Katsutoshi, Nakagiri Yuuhei, Imai Hiroshi, Noga Akira, Obbineni Jagan Mohan, Zimmermann Noemi, Nakajima Masako, Shibata Daisuke, Shibata Misa, Shiba Kogiku, Kita Masaki, Kigoshi Hideo, Tanaka Yui, Yamasaki Yuya, Asahina Yuma, Song Chihong, Nomura Mami, Nomura Mamoru, Nakajima Ayako, Nakachi Mia, Yamada Lixy, Nakazawa Shiori, Sawada Hitoshi, Murata Kazuyoshi, Mitsuoka Kaoru, Ishikawa Takashi, Wakabayashi Ken-Ichi, Kon Takahide, Inaba Kazuo
机构信息
Shimoda Marine Research Center, University of Tsukuba, 5-10-1 Shimoda, Shizuoka 415-0025, Japan.
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan.
出版信息
Sci Adv. 2021 Feb 26;7(9). doi: 10.1126/sciadv.abf3621. Print 2021 Feb.
Light-responsive regulation of ciliary motility is known to be conducted through modulation of dyneins, but the mechanism is not fully understood. Here, we report a novel subunit of the two-headed f/I1 inner arm dynein, named DYBLUP, in animal spermatozoa and a unicellular green alga. This subunit contains a BLUF (sensors of blue light using FAD) domain that appears to directly modulate dynein activity in response to light. DYBLUP (dynein-associated BLUF protein) mediates the connection between the f/I1 motor domain and the tether complex that links the motor to the doublet microtubule. lacking the DYBLUP ortholog shows both positive and negative phototaxis but becomes acclimated and attracted to high-intensity blue light. These results suggest a mechanism to avoid toxic strong light via direct photoregulation of dyneins.
已知纤毛运动的光响应调节是通过动力蛋白的调节来实现的，但其机制尚未完全明确。在此，我们报告了一种在动物精子和单细胞绿藻中发现的双头f/I1内臂动力蛋白的新亚基，命名为DYBLUP。该亚基包含一个BLUF（利用黄素腺嘌呤二核苷酸的蓝光传感器）结构域，似乎能直接响应光来调节动力蛋白的活性。DYBLUP（动力蛋白相关BLUF蛋白）介导f/I1运动结构域与将动力蛋白连接到双联微管的系链复合体之间的连接。缺乏DYBLUP直系同源物的个体既表现出正向光趋性又表现出负向光趋性，但会适应并被高强度蓝光吸引。这些结果提示了一种通过动力蛋白的直接光调节来避免有毒强光的机制。
Zotero 插件