African Swine Fever Unit, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 6-20-1 Josuihoncho, Kodaira, Tokyo, Japan.
Foot and Mouth Disease Unit, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 6-20-1 Josuihoncho, Kodaira, Tokyo, Japan.
Sci Rep. 2021 Feb 26;11(1):4759. doi: 10.1038/s41598-021-84237-2.
African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), a fatal hemorrhagic disease of domestic pigs and wild boar. The virus primarily infects macrophage and monocyte host cells, these do not grow in vitro. Many attempts have been made to establish sustainable ASFV-sensitive cell lines, but which supported only low viral replication levels of limited, mostly artificially attenuated strains of ASFV. Here, we examined the competence of a novel cell line of immortalized porcine kidney macrophages (IPKM) for ASFV infection. We demonstrated that IPKM cells can facilitate high levels (> 10 TCID/mL) of viral replication of ASFV, and hemadsorption reactions and cytopathic effects were observed as with porcine alveolar macrophages when inoculated with virulent field isolates: Armenia07, Kenya05/Tk-1, and Espana75. These results suggested that IPKM may be a valuable tool for the isolation, replication, and genetic manipulation of ASFV in both basic and applied ASF research.
非洲猪瘟病毒（ASFV）是非洲猪瘟（ASF）的病原体，这是一种致命的家猪和野猪出血性疾病。该病毒主要感染巨噬细胞和单核细胞宿主细胞，但这些细胞在体外无法生长。许多人尝试建立可持续的 ASFV 敏感细胞系，但这些细胞系仅支持低水平的病毒复制，且主要是人工减毒的 ASFV 菌株。在这里，我们研究了一种新型的永生化猪肾巨噬细胞（IPKM）细胞系对 ASFV 感染的能力。我们证明，IPKM 细胞可以促进高水平（>10TCID/mL）的 ASFV 复制，并且当接种强毒田间分离株时，如亚美尼亚 07 株、肯尼亚 05/Tk-1 株和西班牙 75 株，会观察到血吸附反应和细胞病变效应。这些结果表明，IPKM 可能是 ASF 基础和应用研究中分离、复制和遗传操作 ASFV 的有价值工具。
