Meints R H, Lee K, Burbank D E, Van Etten J L
Virology. 1984 Oct 30;138(2):341-6. doi: 10.1016/0042-6822(84)90358-1.
Ultrastructural studies revealed that the virus, PBCV-1, adsorbs to the surface of the Chlorella-like green alga NC64A and enzymatically digests a portion of the host cell wall. The viral DNA is then released into the interior of the cell leaving an empty capsid on the surface. Thus uncoating of the viral genome occurs at the surface of its host. PBCV-1 also adsorbs to and digests the host wall of either heat-killed, methanol-extracted, or purified cell wall fragments.
超微结构研究显示,病毒PBCV - 1吸附在类小球藻绿藻NC64A的表面,并通过酶解作用消化宿主细胞壁的一部分。然后病毒DNA释放到细胞内部,在表面留下一个空衣壳。因此,病毒基因组的脱壳发生在宿主表面。PBCV - 1还能吸附并消化热灭活、甲醇提取或纯化的细胞壁片段的宿主细胞壁。