Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Mexico City, Mexico.
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Mexico City, Mexico.
Acta Trop. 2021 May;217:105872. doi: 10.1016/j.actatropica.2021.105872. Epub 2021 Feb 25.
Giardia duodenalis is a parasite that causes a large number of diarrheal diseases around the world. It is noteworthy that in a large number of processes, Giardia requires fewer components than other eukaryotes, even without some organelles such as mitochondria and peroxisomes. Despite this, core histones are known to exist in Giardia and epigenetic marks have been found on them, suggesting that they somehow control the expression of certain genes. The regulation of the expression of ribosomal DNA (rDNA) is essential, since it is required to maintain adequate levels of ribosomes and, given the nature of tandem repeat, it is a feasible area to create genomic instability. In Giardia, it is not known how this process occurs, but as in other eukaryotes, it is suggested through various epigenetic mechanisms. Thus, in the present work we seek to identify how chromatin is distributed through the Giardia rDNA and if there were histone marks that could control its expression.
十二指肠贾第鞭毛虫是一种寄生虫，它在世界范围内引起了大量的腹泻疾病。值得注意的是，在大量的过程中，贾第鞭毛虫比其他真核生物需要更少的成分，甚至没有一些细胞器，如线粒体和过氧化物酶体。尽管如此，已知贾第鞭毛虫中存在核心组蛋白，并且在其上发现了表观遗传标记，这表明它们以某种方式控制某些基因的表达。核糖体 DNA（rDNA）表达的调控是必不可少的，因为它需要维持核糖体的足够水平，并且鉴于串联重复的性质，它是产生基因组不稳定性的可行区域。在贾第鞭毛虫中，尚不清楚这个过程是如何发生的，但与其他真核生物一样，它通过各种表观遗传机制被提出。因此，在本工作中，我们试图确定染色质如何分布在贾第鞭毛虫 rDNA 中，如果有组蛋白标记可以控制其表达。
