School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China.
Forensic Sci Int. 2021 Apr;321:110720. doi: 10.1016/j.forsciint.2021.110720. Epub 2021 Feb 11.
In the field of drowning research, the method of diatom morphology has been most applied to determine whether the cause of death is drowning. However, the characteristics of complex operation, high level of professional knowledge drive us to propose a new method. Here, based on the common phytoplankton in water(such as diatoms and Aeromonas), aiming at the rbcL, 23 S, NIES, rPOD, Hly and preprotoxin aerolysin gene, we designed 6 pairs of specific primers and applied SYBR Green real-time qPCR(RT-qPCR) method to detect phytoplankton in the Pearl River Basin of Guangdong Province, China, so as to achieve the purpose of diagnosing drowning. After the experimental verification of the corresponding algae species and the standard strains of bacteria, as well as the verification of tissue samples (lung, liver and kidney) of 56 cases( 40 drowning cases and 16 non-drowning cases), we found that these primers were of great accuracy and tedious laboratory work of diatom test was reduced. Based on the advantages of high throughput, short period and high sensitivity, this RT-qPCR method is expected to diagnose drowning more rapidly and accurately.
在溺水研究领域，硅藻形态学方法已被广泛应用于确定死亡原因是否为溺水。然而，其操作复杂、专业知识要求高的特点促使我们提出了一种新方法。在这里，我们基于水中常见的浮游植物（如硅藻和气单胞菌），针对 rbcL、23S、NIES、rPOD、Hly 和前原噬菌体 aerolysin 基因，设计了 6 对特异性引物，并应用 SYBR Green 实时 qPCR（RT-qPCR）方法检测中国广东省珠江流域的浮游植物，以达到诊断溺水的目的。在对相应藻类物种和细菌标准菌株进行实验验证以及对 56 例组织样本（肺、肝和肾）进行验证（40 例溺水和 16 例非溺水）后，我们发现这些引物具有很高的准确性，同时减少了硅藻测试的繁琐实验室工作。基于高通量、短周期和高灵敏度的优势，这种 RT-qPCR 方法有望更快速、更准确地诊断溺水。
