利用生物信息学方法鉴定和分析甘薯[Ipomoea batatas (L.) Lam]β-半乳糖苷酶家族基因。

Genome-wide in silico identification and expression analysis of beta-galactosidase family members in sweetpotato [Ipomoea batatas (L.) Lam].

机构信息

Key laboratory of phylogeny and comparative genomics of the Jiangsu province, School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China.

Crop research institute, Shandong Academy of Agricultural Sciences/ Scientific Observing and Experimental Station of Tuber and Root Crops in Huang-Huai-Hai Region, Ministry of Agriculture and Rural Affairs, Jinan, 250100, China.

出版信息

BMC Genomics. 2021 Feb 27;22(1):140. doi: 10.1186/s12864-021-07436-1.

BACKGROUND

Sweetpotato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. β-galactosidase (bgal) is a glycosyl hydrolase involved in cell wall modification, which plays essential roles in plant development and environmental stress adaptation. However, the function of bgal genes in sweetpotato remains unclear.

RESULTS

In this study, 17 β-galactosidase genes (Ibbgal) were identified in sweetpotato, which were classified into seven subfamilies using interspecific phylogenetic and comparative analysis. The promoter regions of Ibbgals harbored several stress, hormone and light responsive cis-acting elements. Quantitative real-time PCR results displayed that Ibbgal genes had the distinct expression patterns across different tissues and varieties. Moreover, the expression profiles under various hormonal treatments, abiotic and biotic stresses were highly divergent in leaves and root.

CONCLUSIONS

Taken together, these findings suggested that Ibbgals might play an important role in plant development and stress responses, which provided evidences for further study of bgal function and sweetpotato breeding.

背景

番薯（Ipomoea batatas（L.）Lam.）是人类重要的食物来源。β-半乳糖苷酶（bgal）是一种参与细胞壁修饰的糖基水解酶，在植物发育和环境胁迫适应中发挥着重要作用。然而，bgal 基因在番薯中的功能尚不清楚。

结果

本研究在番薯中鉴定出 17 个β-半乳糖苷酶基因（Ibbgal），通过种间系统发育和比较分析将其分为 7 个亚家族。Ibbgal 基因启动子区域含有多种应激、激素和光照响应的顺式作用元件。定量实时 PCR 结果显示，Ibbgal 基因在不同组织和品种中的表达模式存在明显差异。此外，叶片和根中激素处理、非生物和生物胁迫下的表达谱差异很大。