School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
Toxicon. 2021 May;195:1-6. doi: 10.1016/j.toxicon.2021.02.014. Epub 2021 Feb 25.
Patulin, one of the most common mycotoxins produced primarily by the Penicillium, Aspergillus and Byssochlamys species, is often associated with fruits and fruit-based products. Biodegradation by microbes is an effective method to remove or detoxify mycotoxins. In this study, a bacterial strain with patulin degradation capability was selectively isolated using oxindole, an analogue to patulin, as the sole carbon source, and identified as Pseudomonas poae JSU-Y1 by phylogenetic analysis on the basis of 16S rRNA sequence. This isolated bacterium could inhibit the growth of Penicillium expansum both on plate medium and apple fruit with inhibition ratio of 30.3% and 44.9%, respectively. Up to 87.7% of the initial patulin (2.5 μg/mL) was removed after incubation with Pseudomonas poae JSU-Y1 in liquid medium at 30 °C for 72 h. When challenged with apple juice, 79% of patulin could be degraded by this isolated strain. Additionally, ascladiol was tentatively identified as the patulin degradation intermediate by LC-MS analysis. Taken together, the experiment results indicated that the isolated Pseudomonas poae JSU-Y1 would be a promising bacterial resource to control patulin contamination and toxigenic fungal growth in agricultural products.
展青霉素是由青霉属、曲霉属和外瓶霉属等真菌产生的最常见的霉菌毒素之一，常与水果及其制品有关。微生物的生物降解是去除或解毒霉菌毒素的有效方法。本研究以展青霉素类似物吲哚-3-乙酸作为唯一碳源，选择性分离出具有展青霉素降解能力的细菌菌株，根据 16S rRNA 序列的系统发育分析，将其鉴定为解淀粉欧文氏菌 JSU-Y1。该分离菌在平板培养基和苹果果实上均可抑制扩展青霉的生长，抑制率分别为 30.3%和 44.9%。解淀粉欧文氏菌 JSU-Y1 在 30°C 下液体培养 72 h 后，可去除初始浓度为 2.5 μg/mL 的展青霉素 87.7%。当用苹果汁进行挑战时，该分离菌株可降解 79%的展青霉素。此外，通过 LC-MS 分析，推测 2-氧吲哚-3-乙酸是展青霉素的降解中间产物。总之，实验结果表明，分离得到的解淀粉欧文氏菌 JSU-Y1 可能是一种有前途的细菌资源，可用于控制农产品中展青霉素污染和产毒真菌的生长。
