Ibrahim Ezzeldin, Nasser Raghda, Hafeez Rahila, Ogunyemi Solabomi Olaitan, Abdallah Yasmine, Khattak Arif Ali, Shou Linfei, Zhang Yang, Ahmed Temoor, Atef Hatamleh Ashraf, Abdullah Al-Dosary Munirah, M Ali Hayssam, Luo Jinyan, Li Bin
State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Department of Vegetable Diseases Research, Plant Pathology Research Institute, Agriculture Research Centre, Giza 12916, Egypt.
Plants (Basel). 2023 Jun 12;12(12):2277. doi: 10.3390/plants12122277.
Some endophyte bacteria can improve plant growth and suppress plant diseases. However, little is known about the potential of endophytes bacteria to promote wheat growth and suppress the seedling blight pathogen . This study was conducted to isolate and identify endophytic bacteria and evaluate their efficacy for the plant growth promotion and disease suppression of seedling blight (FSB) in wheat. The strain CO showed strong antifungal activity in vitro and under greenhouse conditions against strain PH-1. The cell-free supernatants (CFSs) of strain CO were able to inhibit the mycelium growth, the number of colonies forming, spore germination, germ tube length, and the mycotoxin production of FSB with an inhibition rate of 87.00, 62.25, 51.33, 69.29, and 71.08%, respectively, with the highest concentration of CFSs. The results indicated that exhibited multifarious antifungal properties, such as the production of hydrolytic enzymes, siderophores, and lipopeptides. In addition, compared to untreated seeds, wheat plants treated with the strain showed significant growth rates, where root and shoot length increased by about 33% and the weight of fresh roots, fresh shoots, dry roots, and dry shoots by 50%. In addition, the strain produced high levels of indole-3-acetic acid, phosphate solubilization, and nitrogen fixation. Finally, the strain demonstrated strong antagonistic properties as well as a variety of plant growth-promoting properties. Thus, this result suggest that this strain could be used as an alternate to synthetic chemicals, which can serve as an effective method of protecting wheat from fungal infection.
一些内生细菌可以促进植物生长并抑制植物病害。然而，关于内生细菌促进小麦生长和抑制苗枯病病原菌的潜力，人们了解甚少。本研究旨在分离和鉴定内生细菌，并评估它们对小麦苗枯病（FSB）的促生长和抑病效果。菌株CO在体外和温室条件下对菌株PH-1表现出很强的抗真菌活性。菌株CO的无细胞上清液（CFSs）能够抑制FSB的菌丝生长、菌落形成数量、孢子萌发、芽管长度和霉菌毒素产生，在CFSs最高浓度时，抑制率分别为87.00%、62.25%、51.33%、69.29%和71.08%。结果表明，该菌株表现出多种抗真菌特性，如产生水解酶、铁载体和脂肽。此外，与未处理的种子相比，用该菌株处理的小麦植株生长速率显著提高，根和芽长度增加约33%，鲜根、鲜芽、干根和干芽重量增加50%。此外，该菌株还产生高水平的吲哚-3-乙酸、溶解磷和固氮。最后，该菌株表现出很强的拮抗特性以及多种促进植物生长的特性。因此，这一结果表明该菌株可作为合成化学药剂的替代品，这可以作为保护小麦免受真菌感染的有效方法。
