细胞壁多糖解体在拟茎点霉诱导龙眼果实病害发生和软化中的作用。
The role of cell wall polysaccharides disassembly in Lasiodiplodia theobromae-induced disease occurrence and softening of fresh longan fruit.
机构信息
Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou, Fujian 350002, China.
Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou, Fujian 350002, China; College of Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China.
出版信息
Food Chem. 2021 Jul 30;351:129294. doi: 10.1016/j.foodchem.2021.129294. Epub 2021 Feb 15.
Cell wall polysaccharides in fruits act a pivotal role in their resistance to fungal invasion. Lasiodiplodia theobromae (Pat.) Griff. & Maubl. is a primary pathogenic fungus causing the spoilage of fresh longan fruit. In this study, the influences of L. theobromae inoculation on the disassembly of cell wall polysaccharides in pericarp of fresh longans and its association with L. theobromae-induced disease and softening development were investigated. In contrast to the control, samples with L. theobromae infection showed more severe disease development, lower firmness, lower amounts of cell wall materials, covalent-soluble pectin, ionic-soluble pectin, cellulose and hemicellulose, whereas higher value of water-soluble pectin, higher activities of cell wall polysaccharide-disassembling enzymes (cellulase, β-galactosidase, polygalacturonase and pectinesterase). These findings revealed that cell wall polysaccharides disassembly induced by enzymatic manipulation was an essential pathway for L. theobromae to infect harvested longans, and thus led to the disease occurrence and fruit softening.
水果细胞壁多糖在其抵御真菌入侵方面起着关键作用。拟茎点霉（Pat.）格里菲斯和马布尔是导致鲜龙眼果实腐烂的主要病原真菌。本研究探讨了拟茎点霉接种对鲜龙眼果皮细胞壁多糖解体的影响及其与拟茎点霉诱导的病害和软化发展的关系。与对照相比，感染拟茎点霉的样品表现出更严重的病害发展、更低的硬度、更低的细胞壁物质、共价溶性果胶、离子溶性果胶、纤维素和半纤维素含量，而更高的水溶性果胶含量、更高的细胞壁多糖分解酶（纤维素酶、β-半乳糖苷酶、多聚半乳糖醛酸酶和果胶酯酶）活性。这些发现表明，酶促作用引起的细胞壁多糖解体是拟茎点霉感染采后龙眼的一个重要途径，从而导致病害发生和果实软化。
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