Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Food Chem. 2018 May 1;247:16-22. doi: 10.1016/j.foodchem.2017.12.017. Epub 2017 Dec 8.
Effects of Lasiodiplodia theobromae on reactive oxygen species (ROS) production-scavenging system during L. theobromae-induced pericarp browning and disease development of harvested "Fuyan" longans were investigated. Compared with control longans, L. theobromae-inoculated longans exhibited higher pericarp browning index and fruit disease index, higher pericarp O generation rate and MDA content. Moreover, L. theobromae infection also resulted in lower contents of pericarp AsA and GSH, lower levels of pericarp DPPH radical scavenging ability and reducing power. Additionally, L. theobromae infection decreased the activities of pericarp SOD, CAT and APX from day 2 to day 5. These findings suggested that L. theobromae-induced pericarp browning and disease development of harvested longans might be due to reduction of ROS scavenging ability and increase in ROS production, which might stimulate membrane lipid peroxidation, disrupt cellular membrane structure, and cause the loss of cellular compartmentalization and disease resistance, in turn, resulting in pericarp browning and disease development.
研究了鲜荔枝果皮褐变病病原菌胶孢炭疽菌（Lasiodiplodia theobromae）诱导果皮褐变和采后发病过程中活性氧（ROS）产生-清除系统的变化。与对照相比，接种胶孢炭疽菌的荔枝果皮褐变指数和果实发病率更高，果皮 O 生成速率和 MDA 含量更高。此外，胶孢炭疽菌感染还导致果皮抗坏血酸（AsA）和谷胱甘肽（GSH）含量降低，果皮 DPPH 自由基清除能力和还原力降低。同时，接种后第 2 天至第 5 天，果皮中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（APX）活性降低。这些结果表明，胶孢炭疽菌诱导的采后荔枝果皮褐变和发病可能是由于 ROS 清除能力降低和 ROS 产生增加所致，这可能刺激膜脂质过氧化，破坏细胞膜结构，导致细胞区室化和抗病性丧失，进而导致果皮褐变和发病。
