Second Department of Urology, the Fourth Affiliated Hospital of 26488China Medical University, Shenyang City, Liaoning Province, China.
Fourth Clinical College of 26488China Medical University, Shenyang City, Liaoning Province, China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033821995284. doi: 10.1177/1533033821995284.
Prostate cancer is the most commonly diagnosed cancer and second leading cause of cancer death in men. Enoxacin, a third-generation fluoroquinolone antibiotic, was found with anti-proliferative effects against many cancer types. This study was to further investigate its effects against prostate cancer and explore the underlying molecular mechanisms.
PC-3 cells were treated with Enoxacin at different concentrations. Tumor model was established by subcutaneously injecting PC-3 cells into nude mice. MTT assay was used to detect cell viability. ELISA assay, Annexin V/PI staining and TUNEL assay were used to detect apoptosis. RT-qPCR and western blot were used to detect the gene and protein expression, respectively.
Our data showed that Enoxacin inhibited PC-3 cell proliferation and induced the apoptosis through up-regulating the expression of pro-apoptotic proteins, while down-regulating expression levels of anti-apoptotic proteins. Moreover, Enoxacin increased the gene and protein expression of the autophagy and endoplasmic reticulum stress markers. Treating tumor-bearing mice with Enoxacin significantly inhibited tumor growth in xenograft tumor model.
Our results suggested that Enoxacin could be developed as a potential anti-tumor agent against prostate carcinoma.
前列腺癌是男性最常见的癌症，也是癌症死亡的第二大主要原因。恩诺沙星是一种第三代氟喹诺酮类抗生素，已被发现对多种癌症类型具有抗增殖作用。本研究旨在进一步研究其对前列腺癌的作用，并探讨其潜在的分子机制。
用不同浓度的恩诺沙星处理 PC-3 细胞。通过将 PC-3 细胞皮下注射到裸鼠中来建立肿瘤模型。MTT 法检测细胞活力。ELISA 法、Annexin V/PI 染色和 TUNEL 法检测细胞凋亡。RT-qPCR 和 Western blot 分别用于检测基因和蛋白表达。
我们的数据表明，恩诺沙星通过上调促凋亡蛋白的表达，同时下调抗凋亡蛋白的表达水平，抑制 PC-3 细胞增殖并诱导细胞凋亡。此外，恩诺沙星增加了自噬和内质网应激标志物的基因和蛋白表达。在异种移植肿瘤模型中，用恩诺沙星治疗荷瘤小鼠可显著抑制肿瘤生长。
我们的研究结果表明，恩诺沙星可能被开发为一种治疗前列腺癌的潜在抗肿瘤药物。
