Monocyte phenotype as a predictive marker for wound healing in diabetes-related foot ulcers.

AIMS

Delayed healing of diabetes-related foot ulcers (DRFUs) is associated with increased macrophage and matrix metalloproteinases (MMPs) at the wound site. Whether circulating monocyte phenotype and/or MMPs are altered in association with wound healing outcome is unknown, and was investigated in this study.

METHODS

Blood was obtained from 21 participants with DRFU, at initial visit (V1), week-4 (V2), and week-8 (V3) for measurement of monocyte number (CD14), phenotype (CD16, CD163) and chemokine receptors (CCRs) by flow cytometry, and circulating MMPs and TIMP-1 by ELISA.

RESULTS

Six wounds healed during the study. At V1, non-classical CD16 monocytes and MMP-3 were higher in healed vs unhealed (both p < 0.05). At V3, the increased %CD16 persisted and %CCR2 was decreased in healed, but no other monocyte markers nor MMP/TIMP differed between groups. Increased wound closure rate (WCR) at V3 correlated with increased %CD16 monocytes and decreased MMP-2 at V1 or V1 + V2. Receiver operating characteristic (ROC) curves yielded an area-under-the-curve of %CD16 at V1 of 0.78 to predict ulcer healing at V3.

CONCLUSIONS

These results indicate that circulating monocyte phenotype and MMPs alter as DRFUs heal. The relationship of %CD16 monocytes with WCR and ROC curve suggest a predictive role of %CD16 monocytes for ulcer healing.