Nuryady Moh Mirza, Nurcahyo Raden Wisnu, Hindun Iin, Fatmawati Diani
Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang, Malang, Indonesia.
Master Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Vet World. 2021 Jan;14(1):33-39. doi: 10.14202/vetworld.2021.33-39. Epub 2021 Jan 6.
Trypanosomiasis, also known as surra, is an infectious disease with a wide host spectrum. In Indonesia, this disease is caused by . Various trypanocidal drugs have been used to treat this pathogen and subsequent disease. Yet, the long-term trypanocidal administration generates drug-resistant . Some have identified genetic alterations in transporter protein-coding genes that may be responsible for drug resistance. The Multidrug Resistance Protein E (MRPE) gene is a likely candidate gene responsible for the individual resistance. To date, no research has focused on MRPE (MRPE) in this context. Hence, this research aimed at analyzing and characterizing the MRPE gene and protein using a bioinformatics approach.
was isolated from buffalo suffering from surra in Ngawi Regency, Indonesia. Isolated was inoculated and cultured in male mice. The genome was isolated from mouse blood with a parasitemia degree as high as 10. A polymerase chain reaction procedure was conducted to amplify the putative MRPE coding gene. The amplicon was sequenced and analyzed using MEGA X, BLAST, and I-tasser softwares.
The putative MRPE coding gene showed sequence similarity as high as 99.79% against the MRPE gene from . The protein profile and characteristics depicted that the putative MRPE protein was related to a family of Adenosine Triphosphate-Binding Cassette (ABC) transporter proteins. This family of transporter proteins plays a crucial role in the resistance toward several medicines.
The obtained gene sequence in this research was identified as the MRPE. This gene is homologous to the . MRPE gene and possesses ligand active sites for Adenylyl Imidodiphosphate. In addition, MRPE contains enzyme active sites similar to the cystic fibrosis transmembrane conductance regulator. These data suggest that ABC transport proteins, like MRPE, may be necessary to confer trypanocidal drug resistance in .
锥虫病，也称为苏拉病，是一种宿主范围广泛的传染病。在印度尼西亚，这种疾病由……引起。各种杀锥虫药物已被用于治疗这种病原体及后续疾病。然而，长期使用杀锥虫药物会产生耐药性……。一些研究已经确定了转运蛋白编码基因中的基因改变，这些改变可能与耐药性有关。多药耐药蛋白E（MRPE）基因可能是导致个体耐药的候选基因。迄今为止，尚未有研究聚焦于此背景下的MRPE（MRPE）。因此，本研究旨在使用生物信息学方法分析和表征MRPE基因及蛋白。
从印度尼西亚恩加威县患有苏拉病的水牛身上分离……。将分离出的……接种到雄性小鼠体内并进行培养。从寄生虫血症程度高达10的小鼠血液中分离出……基因组。进行聚合酶链反应程序以扩增推定的MRPE编码基因。使用MEGA X、BLAST和I-tasser软件对扩增子进行测序和分析。
推定的MRPE编码基因与来自……的MRPE基因的序列相似性高达99.79%。蛋白质图谱和特征表明，推定的MRPE蛋白与三磷酸腺苷结合盒（ABC）转运蛋白家族有关。这个转运蛋白家族在对几种药物的耐药性中起关键作用。
本研究中获得的基因序列被鉴定为MRPE。该基因与……同源。MRPE基因拥有腺苷酰亚胺二磷酸的配体活性位点。此外，MRPE含有与囊性纤维化跨膜传导调节因子相似的酶活性位点。这些数据表明，像MRPE这样的ABC转运蛋白可能是赋予……杀锥虫药物耐药性所必需的。
