Agrawal Samiksha, Singh Ajay Pratap, Singh Rashmi, Saikia Raktim, Choudhury Soumen, Shukla Amit, Prabhu Shyama N, Agrawal Jitendra
College of Biotechnology, Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidhyalaya Ewam Gau Anusandhan Sansthan Mathura, Uttar Pradesh, India.
Department of Veterinary Microbiology, Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidhyalaya Ewam Gau Anusandhan Sansthan Mathura, Uttar Pradesh, India.
Vet World. 2021 Jan;14(1):200-209. doi: 10.14202/vetworld.2021.200-209. Epub 2021 Jan 23.
Selection and dissemination of plasmid-encoded extended-spectrum β-lactamase (ESBL) among confers resistance to beta-lactam antibiotics. The purpose of this study was to determine the prevalence and molecular characteristics of ESBL-producing organisms isolated from dairy cattle with a uterine infection.
Bacterial isolates (n=62) were characterized by biochemical test for genus and species determination. Antimicrobial susceptibility tests were performed by Kirby-Bauer disk diffusion method using panel of antibiotics for initial screening of ESBL organism. Phenotypic confirmation of ESBL-suspected strains was done by combination disk method and double-disk method. Multiplex polymerase chain reaction (PCR) was carried out for phylogrouping of isolates as well as for genotyping ESBL genes. Enterobacterial repetitive intergenic consensus-PCR method was used for genotypic characterization of isolates.
Antibiotic susceptibility profile of (n=40) isolates showed high rates of resistance for ampicillin (95.0%), cefpodoxime (97.5%), cefotaxime (87.5%), and ceftriaxone (70%). However, low rates of resistance were observed for cefoxitin (25%), amoxicillin/clavulanic acid (20%), ceftazidime (17.5%), gentamicin (10%), and ertapenem (7.5%). A total of 39/40 isolates were confirmed as ESBL with Epsilometer test as well as the genotypic method and 28 (70%) of them were multidrug-resistant. Genotype was observed as a predominant beta-lactamase type with the preponderance of CTX-M Group 1. The following combinations were observed: + in 15 (36.2%) isolates, / in 8 (5.2%) isolates, and / in 6 (5.2%) isolates. The phylogenetic grouping of strains revealed the highest prevalence for B1 (22.0%) followed by A (20%).
This report shows a high frequency of ESBL from cattle with postpartum uterine infections. These isolates showed reduced susceptibility to common antibiotics used for the treatment of uterine infections greater affecting the therapeutic outcome.
质粒编码的超广谱β-内酰胺酶（ESBL）的选择与传播赋予了对β-内酰胺类抗生素的耐药性。本研究的目的是确定从患有子宫感染的奶牛中分离出的产ESBL菌株的流行情况和分子特征。
通过生化试验对细菌分离株（n = 62）进行属和种的鉴定。采用Kirby-Bauer纸片扩散法，使用一组抗生素进行抗菌药敏试验，以初步筛选产ESBL菌株。对疑似ESBL菌株进行表型确认采用组合纸片法和双纸片法。进行多重聚合酶链反应（PCR）对分离株进行系统发育分组以及对ESBL基因进行基因分型。采用肠杆菌重复基因间共识-PCR方法对分离株进行基因分型鉴定。
40株分离株的抗生素药敏谱显示，氨苄西林（95.0%）、头孢泊肟（97.5%）、头孢噻肟（87.5%）和头孢曲松（70%）的耐药率较高。然而，头孢西丁（25%）、阿莫西林/克拉维酸（20%）、头孢他啶（17.5%）、庆大霉素（10%）和厄他培南（7.5%）的耐药率较低。通过Epsilometer试验以及基因分型方法，共确认39/40株分离株为ESBL，其中28株（70%）为多重耐药。观察到基因型为主要的β-内酰胺酶类型，以CTX-M 1组为主。观察到以下组合：15株（36.2%）分离株中blaCTX-M-1 + blaTEM，8株（5.2%）分离株中blaCTX-M-1 / blaSHV，6株（5.2%）分离株中blaCTX-M-1 / blaOXA-1。大肠杆菌菌株的系统发育分组显示，B1组的流行率最高（22.0%），其次是A组（20%）。
本报告显示产后子宫感染奶牛中产ESBL的频率较高。这些分离株对用于治疗子宫感染的常用抗生素的敏感性降低，对治疗结果影响较大。
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