Molecular detection of the gene in from the reproductive tract of Bali cattle with repeat breeder cases on Lombok island.

This study aims to detect which encodes gene from the reproductive tract of Bali cattle with repeat breeder cases. This research was conducted from June to August 2021 using 16 Bali cattle with repeat breeder cases. The reproductive fluids were taken using a plastic sheet gun which was inserted into a Brain Heart Infusion medium, isolated in eosin-methylene blue agar (EMB) and identified using biochemical tests. Antibiotic susceptibility testing of was carried out using the disc diffusion method. Double-disk approximation test was used to screen the presence of which produces Extended-spectrum beta-lactamase. The polymerase chain reaction (PCR) method was used to detect the gene of and sequences of the gene were phylogenetically analyzed. The research results obtained three isolates from 16 reproductive tract fluids of Bali cattle. Antibiotic sensitivity tests showed that 100% of was resistant to penicillin G and oxytetracycline. 66.66% of was resistant to cefotaxime (CTX) and gentamicin, and 33.33% of was resistant to tetracycline. isolates that were resistant to penicillin and CTX showed positive results in the double-disk approximation test. The results of detection using PCR showed that three isolates encoded the gene located at 370 bp on gel electrophoresis. The results of the phylogenetic analysis showed that from the reproductive tract of Bali cattle was related to that encoded 14 isolated from humans.