Kholik Kholik, Srianto Pudji, Aulanni'am Aulanni'am, Madyawati Sri Pantja
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Mataram, Indonesia.
Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
Vet Res Forum. 2024;15(8):397-402. doi: 10.30466/vrf.2024.2014818.4056. Epub 2024 Aug 15.
This study aims to detect which encodes gene from the reproductive tract of Bali cattle with repeat breeder cases. This research was conducted from June to August 2021 using 16 Bali cattle with repeat breeder cases. The reproductive fluids were taken using a plastic sheet gun which was inserted into a Brain Heart Infusion medium, isolated in eosin-methylene blue agar (EMB) and identified using biochemical tests. Antibiotic susceptibility testing of was carried out using the disc diffusion method. Double-disk approximation test was used to screen the presence of which produces Extended-spectrum beta-lactamase. The polymerase chain reaction (PCR) method was used to detect the gene of and sequences of the gene were phylogenetically analyzed. The research results obtained three isolates from 16 reproductive tract fluids of Bali cattle. Antibiotic sensitivity tests showed that 100% of was resistant to penicillin G and oxytetracycline. 66.66% of was resistant to cefotaxime (CTX) and gentamicin, and 33.33% of was resistant to tetracycline. isolates that were resistant to penicillin and CTX showed positive results in the double-disk approximation test. The results of detection using PCR showed that three isolates encoded the gene located at 370 bp on gel electrophoresis. The results of the phylogenetic analysis showed that from the reproductive tract of Bali cattle was related to that encoded 14 isolated from humans.
本研究旨在检测巴厘牛生殖道中与屡配不孕病例相关的编码 基因。本研究于2021年6月至8月进行，使用了16头患有屡配不孕病例的巴厘牛。使用插入脑心浸液培养基的塑料采精枪采集生殖液，在伊红美蓝琼脂（EMB）中分离，并通过生化试验进行鉴定。使用纸片扩散法对 进行抗生素敏感性测试。采用双纸片协同试验筛选产生超广谱β-内酰胺酶的 的存在情况。使用聚合酶链反应（PCR）方法检测 的 基因，并对 基因序列进行系统发育分析。研究结果从16份巴厘牛生殖道液体中获得了三株 分离株。抗生素敏感性测试表明，100%的 对青霉素G和土霉素耐药。66.66%的 对头孢噻肟（CTX）和庆大霉素耐药，33.33%的 对四环素耐药。对青霉素和CTX耐药的 分离株在双纸片协同试验中呈阳性结果。使用PCR进行 检测的结果表明，三株 分离株在凝胶电泳上编码位于370 bp处的 基因。系统发育分析结果表明，巴厘牛生殖道中的 与从人类分离出的编码 的14 相关。
