Shiry Nima, Alavinia Seyed Jalil, Gholamhosseini Amin, Mirvaghefi Alireza
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Vet Res Forum. 2020 Fall;11(4):325-331. doi: 10.30466/vrf.2018.90805.2201. Epub 2020 Dec 15.
The toxicological effects of three commonly used pesticides in Iran on the fries of Caspian kutum (, Kamensky, 1901) were investigated through determining 50.00% lethal concentration (LC) 96hr and cholinesterase (ChE) inhibition. The LC 96-hr of carbaryl, glyphosate, and malathion were equal to 11.69, 6.64, and 0.97 mg L, respectively, that were considered as harmful (10.00 - 100 mg L), toxic (1.00 - 10.00 mg L) and very toxic (< 1.00 mg L) compounds for this species. The exposure of fries to sub-lethal concentrations of the pesticides over 15 days indicated that the average of ChE activity in the head and trunk were 1086.89 ± 124.34 and 627.36 ± 99.60 mU min per mg protein, respectively, with a significant difference relative to each other. There was a significant difference between fry exposed to all three pesticides and the control group in cholinesterase inhibition. The fries exposed to carbaryl (890.12 ± 28.08 mU min per mg protein) and glyphosate (891.77 ± 31.61 mU min per mg protein) showed lower ChE inhibition than those exposed to malathion (790.00 ± 58.14 mU min per mg protein).
通过测定96小时半数致死浓度（LC）和胆碱酯酶（ChE）抑制率，研究了伊朗常用的三种农药对里海库图姆（Caspian kutum，Kamensky，1901）鱼苗的毒理学影响。西维因、草甘膦和马拉硫磷的96小时LC分别为11.69、6.64和0.97毫克/升，对于该物种而言，这些被认为是有害的（10.00 - 100毫克/升）、有毒的（1.00 - 10.00毫克/升）和剧毒的（<1.00毫克/升）化合物。将鱼苗暴露于亚致死浓度的农药15天，结果表明，头部和躯干中ChE活性的平均值分别为每毫克蛋白质1086.89±124.34和627.36±99.60毫微摩尔/分钟，两者之间存在显著差异。在胆碱酯酶抑制方面，暴露于所有三种农药的鱼苗与对照组之间存在显著差异。暴露于西维因（每毫克蛋白质890.12±28.08毫微摩尔/分钟）和草甘膦（每毫克蛋白质891.77±31.61毫微摩尔/分钟）的鱼苗ChE抑制率低于暴露于马拉硫磷（每毫克蛋白质790.00±58.14毫微摩尔/分钟）的鱼苗。
