Masouleh Fatemeh F, Amiri Bagher M, Mirvaghefi Alireza, Ghafoori Hossein, Madsen Steffen S
Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karadj, 31585-4314, Iran.
Department of Biology, Faculty of Science, University of Guilan, Rasht, 41335-19141, Iran.
Environ Monit Assess. 2017 Aug 11;189(9):448. doi: 10.1007/s10661-017-6156-3.
Silver nanoparticles (AgNPs) are increasingly used in several industrial and household products because of their antibacterial and antifungal properties. Hence, there is an inevitable risk that these chemicals may end up in aquatic biotopes and have adverse effects on the fauna. In order to assess potential health effects on aquatic organisms, this study evaluated the effects of waterborne AgNP exposure for 7 days on a set of critical stress parameters in juvenile Caspian kutum (Rutilus kutum), an economically important fish in the Caspian Sea. The applied level 11 μg/l of AgNP is high compared to reported water concentrations and corresponds to 40% of the 96 h LC value, initially determined to be 28 μg/l. Gill heat shock protein 70 (hsp70) mRNA expression, Na/K-ATPase activity and enzymatic activities of liver superoxide dismutase (SOD), glutathione peroxidase (Gpx), lactate dehyrogenase (LDH) and alkaline phosphatase (ALP), and whole-body cortisol and thyroid hormones (T3 and T4) were measured as endpoints. Gill hsp70 mRNA expression increased and gill Na/K-ATPase activity decreased in AgNP-exposed fish compared to controls. The specific activities of all liver enzymes decreased significantly compared to controls. Whole-body cortisol and thyroid hormones decreased compared to controls. In conclusion, the study demonstrates that AgNPs cause oxidative stress and gill osmoregulatory disruption in Caspian kutum juveniles.
由于银纳米颗粒（AgNPs）具有抗菌和抗真菌特性，它们在多种工业和家用产品中的使用越来越广泛。因此，这些化学物质不可避免地可能最终进入水生生物群落，并对动物群产生不利影响。为了评估对水生生物的潜在健康影响，本研究评估了将幼年里海鲟（Rutilus kutum）暴露于含AgNP的水中7天对一系列关键应激参数的影响，里海鲟是里海一种具有重要经济价值的鱼类。所应用的11μg/l的AgNP水平与报告的水体浓度相比很高，相当于最初测定的96小时半数致死浓度（LC）值28μg/l的40%。以鳃热休克蛋白70（hsp70）mRNA表达、钠钾ATP酶活性以及肝脏超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（Gpx）、乳酸脱氢酶（LDH）和碱性磷酸酶（ALP）的酶活性，以及全身皮质醇和甲状腺激素（T3和T4）作为检测终点。与对照组相比，暴露于AgNP的鱼鳃hsp70 mRNA表达增加，鳃钠钾ATP酶活性降低。与对照组相比，所有肝脏酶的比活性均显著降低。与对照组相比，全身皮质醇和甲状腺激素水平降低。总之，该研究表明AgNPs会在幼年里海鲟中引起氧化应激和鳃渗透调节紊乱。
