Zhou Xiaobo, Liu Qiyu, Yuan Wei, Li Zhenhua, Xu Yuliang, Feng Wei, Xu Congjian, Li Fuyou
Department of Chemistry & State Key Laboratory of Molecular Engineering of Polymers & Institute of Biomedicine Science Fudan University Shanghai 200433 China.
Department of Obstetrics and Gynecology of Shanghai Medical School & Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases & Obstetrics and Gynecology Hospital of Fudan University Fudan University Shanghai Shanghai 200011 China.
Adv Sci (Weinh). 2020 Dec 23;8(4):2000441. doi: 10.1002/advs.202000441. eCollection 2021 Feb.
Intraoperative diagnosis of metastatic tumors is of significant importance to the treatment of ovarian cancer. NIR-II fluorescence imaging holds great promise for facile detection of tumor in situ with high sensitivity and resolution. Herein, a kind of NIR-II fluorescent polymer dots (NIR-II Pdots) with high brightness is developed for real-time detection of metastatic ovarian cancer via NIR-II fluorescence imaging. The NIR-II Pdots are constructed via the self-assembly of NIR-II emissive aggregation induced emission luminogens (NIR-II AIEgens) and poly (styrene)-graft-poly(ethylene glycol) in water. Such NIR-II Pdots show very high fluorophore contents of nearly 30% and high quantum yield of 5.4% at emission maximum near 1020 nm. Further modification of the NIR-II Pdots with targeting peptides yields NIR-II Pdots-GnRH, which can afford enhanced affinity of NIR-II Pdots to ovarian cancer. Upon intravenous injection of the NIR-II Pdots, whole-body organs and vessels, peritoneal and lymphatic metastases of ovarian cancer are clearly visualized by NIR-II fluorescence imaging. Under the guidance of NIR-II fluorescence imaging, the metastatic foci with the diameter down to ≈2 mm can be facilely eliminated. The results indicate preclinical potential value of the NIR-II Pdots for metastatic ovarian cancer detection.
术中对转移性肿瘤的诊断对卵巢癌的治疗具有重要意义。近红外二区（NIR-II）荧光成像在原位肿瘤的高灵敏度和高分辨率检测方面具有巨大潜力。在此，通过NIR-II荧光成像开发了一种用于实时检测转移性卵巢癌的高亮度近红外二区荧光聚合物点（NIR-II Pdots）。NIR-II Pdots是通过近红外二区发射性聚集诱导发光剂（NIR-II AIEgens）和聚（苯乙烯）-接枝-聚（乙二醇）在水中的自组装构建而成。这种NIR-II Pdots在1020 nm附近的发射最大值处显示出近30%的非常高的荧光团含量和5.4%的高量子产率。用靶向肽对NIR-II Pdots进行进一步修饰得到NIR-II Pdots-GnRH，其可增强NIR-II Pdots对卵巢癌的亲和力。静脉注射NIR-II Pdots后，通过NIR-II荧光成像可清晰观察到全身器官和血管、卵巢癌的腹膜和淋巴转移。在NIR-II荧光成像的引导下，直径低至≈2 mm的转移灶可轻松消除。结果表明NIR-II Pdots在转移性卵巢癌检测方面具有临床前潜在价值。
