Pennarossa Georgia, Gandolfi Fulvio, Brevini Tiziana A L
Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research, Università degli Studi di Milano, Milan, Italy.
Laboratory of Biomedical Embryology, Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy and Center for Stem Cell Research, Università degli Studi di Milano, Milan, Italy.
Front Cell Dev Biol. 2021 Feb 11;9:646945. doi: 10.3389/fcell.2021.646945. eCollection 2021.
Oocyte-specific competence remains one of the major targets of current research in the field of reproduction. Several mechanisms are involved in meiotic maturation and the molecular signature of an oocyte is considered to reflect its quality and to predict its subsequent developmental and functional capabilities. In the present minireview, we focus on the possible role of mechanotransduction and mechanosensor signaling pathways, namely the Hippo and the RhoGTPase, in the maturing oocyte. Due to the limited access to female gametes, we propose the use of cells isolated from parthenogenetic embryos as a promising model to characterize and dissect the oocyte distinctive molecular signatures, given their exclusive maternal origin. The brief overview here reported suggests a role of the mechanosensing related pathways in oocyte quality and developmental competence and supports the use of uniparental cells as a useful tool for oocyte molecular signature characterization.
卵母细胞特异性能力仍然是生殖领域当前研究的主要目标之一。减数分裂成熟涉及多种机制，卵母细胞的分子特征被认为可以反映其质量，并预测其随后的发育和功能能力。在本综述中，我们重点关注机械转导和机械传感器信号通路，即Hippo和RhoGTPase在成熟卵母细胞中的可能作用。由于获取雌性配子的机会有限，我们建议使用从孤雌生殖胚胎中分离的细胞作为一种有前景的模型，以表征和剖析卵母细胞独特的分子特征，因为它们完全来源于母体。此处报告的简要概述表明，机械传感相关通路在卵母细胞质量和发育能力中发挥作用，并支持使用单亲细胞作为表征卵母细胞分子特征的有用工具。
