Samiei Awat, Tavassoli Mousa, Mardani Karim
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran.
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran.
J Arthropod Borne Dis. 2020 Sep 30;14(3):239-249. doi: 10.18502/jad.v14i3.4557. eCollection 2020 Sep.
Bedbugs are blood feeding ectoparasites of humans and several domesticated animals. There are scarcity of information about the bed bugs population throughout Iran and only very limited and local studies are available. The aim of this study is to assess the phylogenetic relationships and nucleotide diversity using partial sequences of cytochrome oxidase I gene (COI) among the populations of tropical bed bugs inhabiting Iran.
The bedbugs were collected from cities located in different geographical regions of Iran. After DNA extraction PCR was performed for COI gene using specific primers. Then DNA sequencing was performed on PCR products for the all 15 examined samples.
DNA sequencing analysis showed that the all samples were similar, despite the minor nucleotide variations (within the range of 576 to 697bp) on average between 5 and 10 Single nucleotide polymorphisms (SNPs. Subsequently, the results were compared with the database in gene bank which revealed close similarity and sequence homology with other from other parts of the world.
In conclusion, this study has demonstrated the ability of the COI gene to differentiate between the populations from a few different locations in Iran. The current research is the first report of phylogenetic and genetic species diversity analysis conducted on in Iran. These results provided basic information for further studies of molecular epidemiology, public health and pest control operators in Iran.
臭虫是人类和几种家畜的吸血体外寄生虫。伊朗各地关于臭虫种群的信息匮乏，仅有非常有限的局部研究。本研究的目的是利用细胞色素氧化酶I基因（COI）的部分序列评估栖息在伊朗的热带臭虫种群之间的系统发育关系和核苷酸多样性。
从伊朗不同地理区域的城市收集臭虫。DNA提取后，使用特异性引物对COI基因进行PCR扩增。然后对所有15个检测样本的PCR产物进行DNA测序。
DNA测序分析表明，所有样本相似，尽管平均在5至10个单核苷酸多态性（SNP）之间存在微小的核苷酸变异（范围在576至697bp）。随后，将结果与基因库中的数据库进行比较，发现与世界其他地区的其他臭虫具有密切的相似性和序列同源性。
总之，本研究证明了COI基因能够区分来自伊朗几个不同地点的臭虫种群。当前的研究是对伊朗臭虫进行系统发育和遗传物种多样性分析的首次报告。这些结果为伊朗进一步开展分子流行病学、公共卫生和害虫防治研究提供了基础信息。
