Khoshdel-Nezamiha Farahnaz, Vatandoost Hassan, Oshaghi Mohammad Ali, Azari-Hamidian Shahyad, Mianroodi Reza Arabi, Dabiri Farrokh, Bagheri Masoomeh, Terenius Olle, Chavshin Ali Reza
Social Determinants of Health Research Center.
Jpn J Infect Dis. 2016 Jul 22;69(4):319-22. doi: 10.7883/yoken.JJID.2015.269. Epub 2016 Jan 8.
Several mosquito species are vectors of disease; however, to understand their role in disease transmission, accurate species identification is of particular importance. Morphological identification is the main method used, but molecular techniques have emerged as a tool for the identification of closely related species. In this study, mosquitoes from the West Azerbaijan Province in northwestern Iran were characterized on the basis of their rDNA-ITS2 sequences. Nine populations of 6 species of mosquitoes belonging to the genera Anopheles, Culex, Culiseta, and Ochlerotatus were studied. To the best of our knowledge, ITS2 sequences of Culiseta longiareolata and Culex hortensis have been reported for the first time. In addition, ITS2 sequences of Culex theileri and Ochlerotatus caspius have been reported for the first time in Iran. Phylogenetic analysis based on ITS2 showed that subfamilies Anophelinae and Culicinae of the family Culicidae could be differentiated successfully and subgenera Anopheles and Cellia of the genus Anopheles were separated. The analysis showed that the genera Culex, Culiseta, and Ochlerotatus have diverged separately.
几种蚊子是疾病的传播媒介；然而，要了解它们在疾病传播中的作用，准确的物种鉴定尤为重要。形态学鉴定是主要使用的方法，但分子技术已成为鉴定近缘物种的一种工具。在本研究中，对来自伊朗西北部西阿塞拜疆省的蚊子进行了基于其核糖体DNA内转录间隔区2（rDNA-ITS2）序列的特征分析。研究了按蚊属、库蚊属、脉毛蚊属和伊蚊属6种蚊子的9个种群。据我们所知，长鬃脉毛蚊和霍氏库蚊的ITS2序列首次被报道。此外，泰勒库蚊和里海伊蚊的ITS2序列在伊朗首次被报道。基于ITS2的系统发育分析表明，蚊科的按蚊亚科和库蚊亚科能够成功区分，按蚊属的按蚊亚属和塞利按蚊亚属也被分开。分析表明，库蚊属、脉毛蚊属和伊蚊属已经分别分化。
