用含离子释放型预反应玻璃离子（S-PRG）纳米填料对牙齿表面进行抗菌涂层处理。
Antibacterial coating of tooth surface with ion-releasing pre-reacted glass-ionomer (S-PRG) nanofillers.
作者信息
Mayumi Kayoko, Miyaji Hirofumi, Miyata Saori, Nishida Erika, Furihata Tomokazu, Kanemoto Yukimi, Sugaya Tsutomu, Shitomi Kanako, Akasaka Tsukasa
机构信息
Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, N13 W7, Kita-ku, Sapporo, Hokkaido, 060-8586, Japan.
Division of Periodontology and Endodontology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Tobetsu-cho, Ishikari-gun, Hokkaido, 061-0293, Japan.
出版信息
Heliyon. 2021 Feb 2;7(2):e06147. doi: 10.1016/j.heliyon.2021.e06147. eCollection 2021 Feb.
OBJECTIVES
Surface pre-reacted glass-ionomer (S-PRG) fillers release antibacterial borate and fluoride ions. We fabricated nanoscale S-PRG fillers (S-PRG nanofillers) for antibacterial coating of tooth surfaces and assessed the antibacterial effects of this coating in vitro. In addition, we creating a canine model of periodontitis to evaluate the effectiveness of S-PRG nanofiller application on tooth roots and improvement of periodontal parameters.
METHODS
Human dentin blocks were coated with S-PRG nanofiller (average particle size: 0.48 μm) and then characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectrometer (EDX), and ion-releasing test. Antibacterial effects of dentin blocks coated with S-PRG nanofiller were examined using bacterial strains, and Next, we created an experimental model of periodontitis in furcation of premolars of beagle dogs. Then, S-PRG nanofiller coating was applied onto exposed tooth root surfaces. Periodontal parameters, gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment level (CAL), were measured from baseline until 4 weeks. In addition, bone healing was radiographically and histologically examined.
RESULTS
SEM and EDX revealed that S-PRG nanofillers uniformly covered the dentin surface after coating. Dentin blocks coated with S-PRG nanofiller showed ion-releasing property, bacterial growth inhibition, and sterilization effects. In the experimental periodontitis model, S-PRG nanofiller coating significantly reduced clinical inflammatory parameters, such as GI (P < 0.01) and BOP (P < 0.05), compared to uncoated samples. In addition, PPD and CAL significantly decreased by S-PRG nanofiller coating (2 weeks: P < 0.05; 3 and 4 weeks: P < 0.01), suggesting the improvement of periodontitis. Micro-CT and histology revealed that bone healing of furcation defects was enhanced by S-PRG nanofiller coating.
CONCLUSION
S-PRG nanofiller coating provides antibacterial effects to tooth surfaces and improves clinical parameters of periodontitis.
目的
表面预反应玻璃离子（S-PRG）填料可释放抗菌硼酸盐和氟离子。我们制备了用于牙齿表面抗菌涂层的纳米级S-PRG填料（S-PRG纳米填料），并在体外评估了该涂层的抗菌效果。此外，我们建立了犬牙周炎模型，以评估S-PRG纳米填料应用于牙根的有效性以及牙周参数的改善情况。
方法
用人牙本质块涂覆S-PRG纳米填料（平均粒径：0.48μm），然后通过扫描电子显微镜（SEM）、能量色散X射线光谱仪（EDX）和离子释放试验进行表征。使用细菌菌株检测涂覆S-PRG纳米填料的牙本质块的抗菌效果，接下来，我们在比格犬前磨牙分叉处建立了牙周炎实验模型。然后，将S-PRG纳米填料涂层应用于暴露的牙根表面。从基线到4周测量牙周参数，牙龈指数（GI）、探诊出血（BOP）、探诊袋深度（PPD）和临床附着水平（CAL）。此外，通过影像学和组织学检查骨愈合情况。
结果
SEM和EDX显示，涂覆后S-PRG纳米填料均匀覆盖在牙本质表面。涂覆S-PRG纳米填料的牙本质块具有离子释放特性、细菌生长抑制和杀菌作用。在实验性牙周炎模型中，与未涂覆的样本相比，S-PRG纳米填料涂层显著降低了临床炎症参数，如GI（P<0.01）和BOP（P<0.05）。此外，S-PRG纳米填料涂层使PPD和CAL显著降低（2周：P<0.05；3周和4周：P<0.01），表明牙周炎有所改善。显微CT和组织学显示，S-PRG纳米填料涂层增强了分叉缺损处的骨愈合。
结论
S-PRG纳米填料涂层为牙齿表面提供抗菌作用，并改善牙周炎的临床参数。
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