使用蛋白质结合微阵列研究NFATc2的REL结构域与五种DNA的结合情况。

REL Domain of NFATc2 Binding to Five Types of DNA Using Protein Binding Microarrays.

作者信息

Ray Sreejana, Tillo Desiree, Durell Stewart R, Khund-Sayeed Syed, Vinson Charles

机构信息

Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Building 37, Room 5000, Bethesda, Maryland 20892, United States.

Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Building 37, Room 5000, Bethesda, Maryland 20892, United States.

出版信息

ACS Omega. 2021 Feb 2;6(6):4147-4154. doi: 10.1021/acsomega.0c04069. eCollection 2021 Feb 16.

NFATc2 is a DNA binding protein in the Rel family transcription factors, which binds a motif better when both cytosines in the dinucleotide are methylated. Using protein binding microarrays (PBMs), we examined the DNA binding of NFATc2 to three additional types of DNA: single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with either 5-methylcytosine (5mC, ) or 5-hydroxymethylcytosine (5hmC, ) in one strand and a cytosine in the second strand. , the complement of the core motif, is better bound as ssDNA compared to dsDNA. dsDNA containing the 5-mer with either 5mC or 5hmC in one DNA strand is bound stronger than . In contrast, the reverse complement is bound weaker when it contains 5mC. Analysis of the available NFATc2:dsDNA complexes rationalizes these PBM data.

NFATc2是Rel家族转录因子中的一种DNA结合蛋白，当二核苷酸中的两个胞嘧啶都被甲基化时，它与一个基序的结合更好。我们使用蛋白质结合微阵列（PBM）研究了NFATc2与另外三种类型DNA的结合：单链DNA（ssDNA）和双链DNA（dsDNA），其中一条链含有5-甲基胞嘧啶（5mC）或5-羟甲基胞嘧啶（5hmC），另一条链含有一个胞嘧啶。与dsDNA相比，作为ssDNA时，核心基序的互补序列结合得更好。在一条DNA链中含有5mC或5hmC的包含5聚体的dsDNA比结合得更强。相反，当它含有5mC时，反向互补序列结合较弱。对现有的NFATc2:dsDNA复合物的分析使这些PBM数据合理化。