Hu Bosen, Chen Lin, Chen Yong, Zhang Zhuo, Wang Xiaohong, Zhou Bo
School of Public Health, Shenyang Medical College, 146 North Huanghe Street, Shenyang, Liaoning 110034, China.
Central Hospital Affiliated to Shenyang Medical College, 5 South 7th West Rd, Shenyang, Liaoning 110024, China.
ACS Omega. 2021 Feb 11;6(7):4759-4766. doi: 10.1021/acsomega.0c05603. eCollection 2021 Feb 23.
Osteoporosis, characterized by a gradual decrease in the number of osteoblasts and a gradual increase in bone resorption of osteoclasts in bone tissue, is a global chronic disease, which severely impairs the quality of life of the elderly. Therefore, it is extremely urgent to study the prevention and treatment of osteoporosis. It has been reported that anthocyanins can regulate bone metabolism and prevent osteoporosis. Cyanidin-3--glucoside (C3G), the most common type of anthocyanin in nature, widely exists in a variety of vegetables and fruits. Although it has been shown that C3G has multiple effects on osteoclasts, its impact(s) and underlying mechanism(s) on osteoblasts are still not clear. Here, we evaluated the effect of C3G on cell proliferation and differentiation of osteoblasts (extracted from the hip joint of patients with osteoporosis) and MC3T3-E1 (a kind of osteoblast cell line from mice). We also test the ability of osteoblasts to mineralize after C3G treatment. To find the underlying mechanism of the above effects, we further evaluated the role of the ERK signaling pathway in C3G regulation of osteoblasts. The results showed that C3G treatment enhanced osteoblast proliferation rate, osteoblast mineralization points, the mRNA levels and protein expression levels of OC (osteocalcin), and the level of ERK phosphorylation, which could be blocked by pretreatment with ERK signaling pathway inhibitor. The above results not only indicate that the ERK pathway was involved in C3G regulation of osteoblast differentiation but also provide strong suggestive evidence that osteoblasts may be promising targets in preventive and therapeutic strategies for osteoporosis.
骨质疏松症是一种全球性的慢性疾病，其特征是骨组织中成骨细胞数量逐渐减少，破骨细胞的骨吸收逐渐增加，严重损害老年人的生活质量。因此，研究骨质疏松症的防治迫在眉睫。据报道，花青素可以调节骨代谢并预防骨质疏松症。矢车菊素-3-O-葡萄糖苷（C3G）是自然界中最常见的花青素类型，广泛存在于多种蔬菜和水果中。虽然已经表明C3G对破骨细胞有多种作用，但其对成骨细胞的影响及其潜在机制仍不清楚。在此，我们评估了C3G对成骨细胞（从骨质疏松症患者的髋关节中提取）和MC3T3-E1（一种来自小鼠的成骨细胞系）的细胞增殖和分化的影响。我们还测试了C3G处理后成骨细胞的矿化能力。为了找到上述作用的潜在机制，我们进一步评估了ERK信号通路在C3G对成骨细胞调节中的作用。结果表明，C3G处理提高了成骨细胞增殖率、成骨细胞矿化点、骨钙素（OC）的mRNA水平和蛋白表达水平以及ERK磷酸化水平，而ERK信号通路抑制剂预处理可阻断这些作用。上述结果不仅表明ERK通路参与了C3G对成骨细胞分化的调节，还提供了有力的提示性证据，即成骨细胞可能是骨质疏松症预防和治疗策略中有前景的靶点。
