Chen Lin, Hu Bosen, Wang Xiaohong, Chen Yong, Zhou Bo
School of Public Health, Shenyang Medical College, Shenyang, China.
Central Hospital Affiliated to Shenyang Medical College, Shenyang, China.
Front Nutr. 2022 Sep 30;9:995643. doi: 10.3389/fnut.2022.995643. eCollection 2022.
Cyanidin-3-O-glucoside (C3G) is the most widely distributed anthocyanin and it can reportedly reduce the risk of osteoporosis, but the molecular mechanism by which C3G promotes bone formation is poorly understood. In the current study, RNA sequencing (RNA-seq) was used to investigate the mechanism of action of C3G in osteogenesis. MC3T3-E1 mouse osteoblasts were divided into a C3G (100 μmol/L)-treated group and a vehicle-treated control group, and differentially expressed genes (DEGs) in groups were evaluated RNA-seq analysis. The functions of the DEGs were evaluated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, and the genes were validated by quantitative real-time PCR. The RNA-seq analysis identified 34 genes that were upregulated in C3G-treated cells compared to vehicle-treated cells, and 17 that were downregulated GO and KEGG pathway analyses indicated that these genes were highly enriched in functions related to lysosomes and glycolipid biosynthesis, among others. The differential expression of ATPase H+-transporting V0 subunit C (), chemokine (C-X3-C motif) ligand 1 (), and lymphocyte antigen 6 complex, locus A () genes was validated by quantitative real-time-PCR. Because these genes have been previously implicated in osteoporosis, they are potential target genes of C3G action in MC3T3-E1 cells. These results provide molecular level evidence for the therapeutic potential of C3G in the treatment of osteoporosis and other disorders of bone metabolism.
矢车菊素 - 3 - O - 葡萄糖苷（C3G）是分布最广泛的花青素，据报道它可以降低骨质疏松症的风险，但C3G促进骨形成的分子机制尚不清楚。在本研究中，使用RNA测序（RNA - seq）来研究C3G在成骨过程中的作用机制。将MC3T3 - E1小鼠成骨细胞分为C3G（100μmol/L）处理组和溶剂处理对照组，并通过RNA - seq分析评估两组中的差异表达基因（DEG）。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析评估DEG的功能，并通过定量实时PCR验证这些基因。RNA - seq分析确定，与溶剂处理细胞相比，C3G处理细胞中有34个基因上调，17个基因下调。GO和KEGG通路分析表明，这些基因在与溶酶体和糖脂生物合成等相关的功能中高度富集。通过定量实时PCR验证了ATP酶H⁺转运V0亚基C、趋化因子（C - X3 - C基序）配体1和淋巴细胞抗原6复合物A位点基因的差异表达。由于这些基因先前已与骨质疏松症有关，它们是C3G在MC3T3 - E1细胞中作用的潜在靶基因。这些结果为C3G在治疗骨质疏松症和其他骨代谢紊乱方面的治疗潜力提供了分子水平的证据。
