Neuroscience Division, Garvan Institute of Medical research, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010 Australia.
St. Vincent's Clinical School, UNSW Sydney, Sydney, NSW, Australia.
STAR Protoc. 2021 Feb 5;2(1):100330. doi: 10.1016/j.xpro.2021.100330. eCollection 2021 Mar 19.
Substances are commonly administered orally in mouse experiments. Here, I describe how to voluntarily administer substances orally in a time- and dose-controlled manner to laboratory mice. This minimizes injury potential and stress often associated with the commonly used intragastric gavage technique. Here, the drug is incorporated into artificially sweetened and flavored jelly and given to mice previously trained to eat the jelly. This can be used for acute and chronic oral drug treatment or for oral glucose tolerance tests. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2010) and Cox et al. (2010).
在小鼠实验中，通常经口给予物质。在此，我将描述如何以时间和剂量可控的方式自愿给予实验室小鼠经口给予物质。这最大限度地减少了与常用的胃内灌胃技术相关的潜在损伤和应激。在此，将药物掺入人工加糖和调味的果冻中，并给予之前已接受过吃果冻训练的小鼠。这可用于急性和慢性口服药物治疗或口服葡萄糖耐量试验。有关该方案的使用和执行的完整详细信息，请参阅 Zhang 等人（2010 年）和 Cox 等人（2010 年）。
