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皮下淀粉样瘤模型用于体内筛选潜在抗纤维蛋白溶解剂。

Subcutaneous amyloidoma models for screening potential anti-fibrillating agents in vivo.

机构信息

Division of Cell Biology and Physiology, CSIR-Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata, West Bengal 700032, India.

Department of Biophysics, Bose Institute, Kolkata, West Bengal 700054, India.

出版信息

STAR Protoc. 2021 Dec 14;2(4):101027. doi: 10.1016/j.xpro.2021.101027. eCollection 2021 Dec 17.

DOI:10.1016/j.xpro.2021.101027
PMID:34977673
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8683767/
Abstract

Here, we describe a robust protocol using mouse models to screen potential insulin-stabilizers and insulin moieties. We have generated a mouse model of amyloidoma, found in diabetic patients undergoing insulin therapy. This model can be used to screen potential insulin stabilizers and insulin moieties to prevent amyloidoma formation. This protocol can further be used for the preclinical validation of therapeutically relevant insulin stabilizers and formulations. The protocol highlights all the critical steps for generating amyloidoma in a preclinical model. For complete details on the use and execution of this profile, please refer to Mukherjee et al. (2021).

摘要

在这里，我们描述了一种使用小鼠模型筛选潜在胰岛素稳定剂和胰岛素片段的可靠方案。我们已经建立了一种在接受胰岛素治疗的糖尿病患者中发现的淀粉样瘤的小鼠模型。该模型可用于筛选潜在的胰岛素稳定剂和胰岛素片段，以防止淀粉样瘤的形成。该方案还可用于具有治疗意义的胰岛素稳定剂和配方的临床前验证。该方案突出了在临床前模型中生成淀粉样瘤的所有关键步骤。有关此方案的使用和执行的完整详细信息，请参阅 Mukherjee 等人。（2021 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/1f80912e2ed9/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/c0ad825328f4/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/b06764daabd3/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/507529a42b16/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/e9924c38ead5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/c878f2a01222/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/1f80912e2ed9/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/c0ad825328f4/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/b06764daabd3/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/507529a42b16/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/e9924c38ead5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/c878f2a01222/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31c/8683767/1f80912e2ed9/gr5.jpg

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本文引用的文献

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Prion-derived tetrapeptide stabilizes thermolabile insulin via conformational trapping.朊病毒衍生的四肽通过构象捕获使热不稳定胰岛素稳定。
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淀粉样纤维的极端黏附活性导致皮下胰岛素抵抗。
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J Diabetes Sci Technol. 2018 Jul;12(4):839-841. doi: 10.1177/1932296817747292. Epub 2017 Dec 21.
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Isolated insulin-derived amyloidoma of the breast.孤立性乳腺胰岛素源性淀粉样瘤
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Selective enhancement of insulin sensitivity in the mature adipocyte is sufficient for systemic metabolic improvements.成熟脂肪细胞中胰岛素敏感性的选择性增强足以改善全身代谢。
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Generating local amyloidosis in mice by the subcutaneous injection of human insulin amyloid fibrils.通过皮下注射人胰岛素淀粉样纤维在小鼠中产生局部淀粉样变性。
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Regulation of insulin resistance and adiponectin signaling in adipose tissue by liver X receptor activation highlights a cross-talk with PPARγ.肝脏X受体激活对脂肪组织中胰岛素抵抗和脂联素信号的调节突出了与过氧化物酶体增殖物激活受体γ的相互作用。
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