Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
Key Laboratory of Neuropharmacology and Translational Medicine of Zhejiang Province, Zhejiang Chinese Medical University, Hangzhou, China.
CNS Neurosci Ther. 2022 Feb;28(2):206-217. doi: 10.1111/cns.13623. Epub 2021 Feb 28.
Noninvasive music adjuvant therapy shows great potential in improving seizure control when combined with routine antiepileptic drugs. However, the diversity of previous music protocols has resulted in disparate outcomes. The optimized protocol and features for music adjuvant therapy are still not fully understood which limits its feasibility.
By applying different regimens of music therapy in various temporal lobe epilepsy models, we evaluated the effect of music in combination with sub-dose drugs on epileptic seizures to determine the optimized protocol.
A subgroup of kindled mice that were responsive to music adjuvant therapy was screened. In those mice, sub-dose drugs which were noneffective on kindled seizures, alleviated seizure severity after 12 h/day Mozart K.448 for 14 days. Shorter durations of music therapy (2 and 6 h/day) were ineffective. Furthermore, only full-length Mozart K.448, not its episodes or other music varieties, was capable of enhancing the efficacy of sub-dose drugs. This music therapeutic effect was not due to increasing cerebral drug concentration, but instead was related with the modulation of seizure electroencephalogram (EEG) spectral powers in the hippocampus.
These results indicate that long-term full-length Mozart K.448 could enhance the anti-seizure efficacy of sub-dose drugs and may be a promising noninvasive adjuvant therapy for temporal lobe epilepsy.
当与常规抗癫痫药物联合使用时，非侵入性音乐辅助疗法在改善癫痫发作控制方面显示出巨大的潜力。然而，由于之前的音乐方案多样化，导致结果存在差异。音乐辅助疗法的优化方案和特征仍未完全了解，这限制了其可行性。
通过在各种颞叶癫痫模型中应用不同的音乐治疗方案，我们评估了音乐与亚剂量药物联合使用对癫痫发作的影响，以确定最佳方案。
筛选出对音乐辅助治疗有反应的亚组点燃小鼠。在这些小鼠中，对点燃性癫痫发作无效的亚剂量药物在接受莫扎特 K.448 音乐治疗 14 天，每天 12 小时后，可减轻癫痫发作的严重程度。较短时间的音乐治疗（每天 2 小时和 6 小时）则无效。此外，只有完整的莫扎特 K.448，而不是其片段或其他音乐类型，能够增强亚剂量药物的疗效。这种音乐治疗效果不是由于增加了大脑中的药物浓度，而是与调节海马中的癫痫脑电图（EEG）频谱功率有关。
这些结果表明，长期的全时长莫扎特 K.448 可能增强亚剂量药物的抗癫痫作用，并且可能是颞叶癫痫的一种有前途的非侵入性辅助治疗方法。
