College of Nursing, University of Nebraska Medical Center, Omaha, NE, USA.
Department of Cellular and Integrative Physiology, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA.
J Matern Fetal Neonatal Med. 2022 Dec;35(25):5513-5519. doi: 10.1080/14767058.2021.1887121. Epub 2021 Feb 28.
Dysregulation of inflammatory processes is linked to perinatal complications yet a comprehensive description of cytokine levels throughout the perinatal period is lacking. We report prospective, serial levels of 29 unique cytokines measured in maternal blood during pregnancy, in the cord blood at birth, and in the neonatal blood.
Pregnant women ( = 140) for recruited from a Midwest tertiary medical center. Blood was obtained at five timepoints: 12-20 weeks, 24-28 weeks, and at labor in the women, umbilical cord at birth, 24-72 h in the newborn. Cytokine levels were analyzed using an electrochemiluminescence-based immunoassay.
Levels for 29 cytokines were measured. The data were separated into two groups: pregnancies with ( = 82) and without major complications ( = 53) (preterm birth, preeclampsia, diabetes mellitus). Eighteen cytokines showed significant changes over time ( < .002). The majority of the cytokines were highest in the newborn. No differences in cytokine levels between complication groups were noted at any timepoint.
This is the first known study to report prospective, serial cytokine levels throughout the perinatal period for pregnancies with/without complications. No differences in maternal cytokine levels between those with/without complications were detected; studies with a larger sample size would be needed to validate our current findings. Results also suggest cytokine dysregulation may be more localized to the placenta making it difficult to measure and predict during pregnancy using maternal systemic blood specimens.
炎症过程的失调与围产期并发症有关，但缺乏对围产期细胞因子水平的全面描述。我们报告了前瞻性、系列性地测量了 140 名孕妇在怀孕期间、分娩时脐带血和新生儿血液中的 29 种独特细胞因子的水平。
从中西部三级医疗中心招募了孕妇（ = 140）。在五个时间点采集血液：12-20 周、24-28 周、妇女分娩时、脐带出生时、新生儿 24-72 小时。使用基于电化学发光的免疫分析法分析细胞因子水平。
测量了 29 种细胞因子的水平。将数据分为两组：有（ = 82）和无主要并发症（ = 53）（早产、子痫前期、糖尿病）的妊娠。18 种细胞因子随时间显著变化（ < .002）。大多数细胞因子在新生儿中含量最高。在任何时间点，并发症组之间的细胞因子水平均无差异。
这是第一项已知的研究，报告了前瞻性、系列性地测量了有/无并发症的妊娠期间围产期的细胞因子水平。在有/无并发症的孕妇中，母体细胞因子水平没有差异；需要更大的样本量研究来验证我们目前的发现。结果还表明，细胞因子失调可能更局限于胎盘，使用母体系统血液样本在怀孕期间进行测量和预测较为困难。
