Department of Biomedical Sciences & Public Health, Polytechnic University of Marche, Ancona, Italy.
Clinic of Dermatology, Department of Clinical & Molecular Sciences, Polytechnic University of Marche, Ancona, Italy.
Future Microbiol. 2021 Mar;16:221-227. doi: 10.2217/fmb-2020-0204. Epub 2021 Mar 1.
The global spread of antibiotic resistance represents a serious threat for public health. We evaluated the efficacy of the antimicrobial peptide LL-37 as antimicrobial agent against multidrug-resistant . LL-37 showed good activity against -1 carrying, extended spectrum β-lactamase- and carbapenemase-producing (minimum inhibitory concentration, MIC, from 16 to 64 mg/l). Checkerboard assays demonstrated synergistic effect of LL-37/colistin combination against all tested strains, further confirmed by time-kill and post antibiotic effect assays. MIC and sub-MIC concentrations of LL-37 were able to reduce biofilm formation. Our preliminary data indicated that LL-37/colistin combination was effective against multidrug-resistant strains and suggested a new possible clinical application.
抗生素耐药性的全球传播对公共健康构成了严重威胁。我们评估了抗菌肽 LL-37 作为抗微生物剂对多药耐药菌的疗效。LL-37 对携带-1、扩展谱β-内酰胺酶和碳青霉烯酶的产酶菌表现出良好的活性（最低抑菌浓度，MIC 为 16 至 64mg/l）。棋盘试验显示 LL-37/黏菌素联合用药对所有测试菌株均具有协同作用，时效和抗生素后效应试验进一步证实了这一点。MIC 和亚 MIC 浓度的 LL-37 能够减少生物膜的形成。我们的初步数据表明，LL-37/黏菌素联合用药对多药耐药菌有效，并提示了一种新的可能的临床应用。
