在土耳其急性弛缓性麻痹监测中检测非脊灰肠道病毒和脊灰肠道病毒。

Detection of non-polio and polio enteroviruses in Acute Flaccid Paralysis surveillance in Turkey.

机构信息

1Ministry of Health, General Directorate of Public Health, Virology Reference and Research Laboratory, Ankara, Turkey.

2Ministry of Health, General Directorate of Public Health, Vaccine Preventable Diseases Department, Ankara, Turkey.

出版信息

Acta Microbiol Immunol Hung. 2021 Mar 1;68(2):92-98. doi: 10.1556/030.2021.01353. Print 2021 Jun 21.

DOI:10.1556/030.2021.01353

PMID:33646138

Abstract

Poliomyelitis was a disease feared worldwide, striking suddenly and paralysing mainly children for life. Monitoring of suspected cases of poliomyelitis is carried out with Acute Flaccid Paralysis (AFP) surveillance in Turkey. This study examines national data of AFP surveillance and the epidemiology of enteroviruses (EV) in Turkey from 2000 to 2019 and gives an overview of the detected serotypes of EVs. A total of 13,640 samples collected from patients with 5216 AFP pre-diagnosed cases (2 samples from each patient) and 3,208 contacts, during a 20-year period (2000-2019) were investigated. All isolated polioviruses were tested for their wild or vaccine origin according to the WHO recommended protocol by PCR and sequencing analysis were performed. Enterovirus positivity was detected in a total of 915 cases, which were identified as 204 Sabin-like polio virus (SLPV) and 711 non-polio enterovirus (NPEV). Of the 204 SLPV, 141 (69.1%) AFP were detected in patients and 63 (30.9%) were detected in samples taken from their contacts. Of the 711 NPEVs, 516 (72.5%) were from AFP cases and 195 (27.5%) were detected in samples taken from their contacts. It is concluded that the reason for the higher detection rate of NPEV in samples from AFP pre-diagnosed cases is attributed to the polio vaccination rates reaching 97% between 2008 and 2019 in Turkey. The most frequently detected NPEV serotypes were Coxackie A24, B3, and Echo 30. This retrospective study is the first comprehensive study in Turkey to evaluate the results of the AFP surveillance in the last 20 years.

摘要

脊髓灰质炎是一种令全世界恐惧的疾病，它突然发作，主要使儿童终生瘫痪。土耳其通过急性弛缓性麻痹（AFP）监测来监测脊髓灰质炎疑似病例。本研究回顾性分析了 2000 年至 2019 年土耳其的 AFP 监测和肠病毒（EV）流行病学数据，并概述了所检测到的 EV 血清型。在 20 年期间（2000-2019 年），共从 5216 例 AFP 预诊断病例（每位患者采集 2 份样本）和 3208 名接触者的 13640 份样本中采集了样本。根据世界卫生组织推荐的方案，对所有分离出的脊髓灰质炎病毒进行了野生或疫苗来源的检测，并通过 PCR 和测序分析进行了检测。总共在 915 例样本中检测到肠道病毒阳性，其中包括 204 例类似萨宾的脊髓灰质炎病毒（SLPV）和 711 例非脊髓灰质炎肠道病毒（NPEV）。在 204 例 SLPV 中，141 例（69.1%）在患者中检测到 AFP，63 例（30.9%）在其接触者的样本中检测到 AFP。在 711 例 NPEV 中，516 例（72.5%）来自 AFP 病例，195 例（27.5%）在其接触者的样本中检测到。得出的结论是，在 AFP 预诊断病例样本中 NPEV 检测率较高的原因是 2008 年至 2019 年期间土耳其脊髓灰质炎疫苗接种率达到 97%。检测到的最常见的 NPEV 血清型是柯萨奇病毒 A24、B3 和埃可 30。这项回顾性研究是土耳其首次对过去 20 年 AFP 监测结果进行的全面评估。