Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
Int J Infect Dis. 2021 May;106:36-40. doi: 10.1016/j.ijid.2021.03.057. Epub 2021 Mar 23.
Acute Flaccid Paralyses Surveillance (AFPS) monitors the emergence of polioviruses and can track Non-Polio Enteroviruses (NPEVs). We report AFPS activity in the Lombardy region (Northern Italy) from 2016 to 2018.
Fecal and respiratory samples were collected from children <15 years who met the WHO definition of an AFP case, analyzed by virus isolation in cell cultures (RD/L20B) and by a one-step real-time RT-PCR assay specific for the 5'-noncoding-region of NPEV. NPEV-positive specimens were further analyzed by sequencing a fragment of the VP1 gene.
36 AFP cases (89 stool and 32 respiratory samples) were reported with an incidence of 1.1/100'000, 0.9/100'000, 0.6/100'000 children <15 years in 2016, 2017, 2018, respectively. Poliovirus was not identified, whereas NPEVs were detected in 19.4% (7/36) of AFP cases. The presence of one Echovirus-25 (2016), two EV- and D68 (2016 and 2018), one EV-A71 (2016), and one Echovirus-30 (2016) sharing high nucleotide identity with NPEVs detected in Europe was identified.
The absence of polio was confirmed. The unpredicted detection of emerging EV-D68, EV-A71, and E-30 sharing high sequence nucleotide similarity with viruses involved in the latest outbreaks, provided valuable and up-to-date information, emphasizing the importance of monitoring NPEVs through AFPS.
急性弛缓性麻痹监测（AFPS）可监测脊灰病毒的出现，并可追踪非脊灰肠道病毒（NPEV）。我们报告了 2016 年至 2018 年意大利北部伦巴第地区的 AFPS 活动情况。
采集符合世界卫生组织急性弛缓性麻痹病例定义的<15 岁儿童的粪便和呼吸道样本，在 RD/L20B 细胞培养物中进行病毒分离，并使用针对 NPEV 5'-非编码区的一步实时 RT-PCR 检测进行分析。对 NPEV 阳性标本进行进一步分析，测序 VP1 基因片段。
共报告了 36 例 AFP 病例（89 份粪便和 32 份呼吸道样本），发病率分别为 2016 年、2017 年和 2018 年<15 岁儿童的 1.1/100000、0.9/100000 和 0.6/100000。未鉴定出脊灰病毒，但在 36 例 AFP 病例中的 19.4%（7/36）中检测到 NPEV。鉴定出一种肠病毒-25（2016 年）、两种肠病毒-D68（2016 年和 2018 年）、一种肠病毒-A71（2016 年）和一种肠病毒-30（2016 年），它们与欧洲检测到的 NPEV 具有高度核苷酸同源性。
确认无脊灰病毒。出人意料地检测到新兴的 EV-D68、EV-A71 和 E-30，它们与最近爆发疫情中涉及的病毒具有高度序列核苷酸相似性，提供了有价值的最新信息，强调了通过 AFPS 监测 NPEV 的重要性。
