利用 DNA 框架对二聚体 i-motif 的折叠协同性进行编程，用于检测小的 pH 变化。

Programming folding cooperativity of the dimeric i-motif with DNA frameworks for sensing small pH variations.

机构信息

Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

出版信息

Chem Commun (Camb). 2021 Apr 4;57(26):3247-3250. doi: 10.1039/d1cc00266j. Epub 2021 Mar 1.

DOI:10.1039/d1cc00266j

PMID:33646233

Abstract

The response sensitivity of a molecular sensor is determined by the folding cooperativity of its responsive module. Using an H-responsive dimeric DNA i-motif as a model, we demonstrate the enhancement of its folding cooperativity through preorganization by a DNA framework, and with it we fabricate robust intracellular pH sensors with high response sensitivity.

摘要

分子传感器的响应灵敏度取决于其响应模块的折叠协同性。本文以 H 响应的二聚体 DNA i-motif 为模型，通过 DNA 框架的预组织来增强其折叠协同性，并以此构建了具有高响应灵敏度的稳健型细胞内 pH 传感器。